Difference between revisions of "CheB"
(→Expression and regulation) |
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * not essential for pellicle biofilm formation and mutant has similar fitness to the wild-type strain when competed during pellicle formation {{PubMed|26122431}} | ||
=== Database entries === | === Database entries === | ||
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* '''Mutant:''' | * '''Mutant:''' | ||
| + | ** DS7306 (marker-less in NCIB3610) {{PubMed|25313396}} | ||
| + | ** TB19 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}} | ||
| + | ** TB213 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}} | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
| Line 142: | Line 146: | ||
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
| + | * [[Daniel Kearns]] | ||
=Your additional remarks= | =Your additional remarks= | ||
| Line 149: | Line 154: | ||
<pubmed> 20122866 </pubmed> | <pubmed> 20122866 </pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>10632876,12123464,1905718,14731274,17850253,14651647, 9657996,8157612,15175317 11553614 21515776 24386445</pubmed> | + | <pubmed>25313396, 26122431, 10632876,12123464,1905718,14731274,17850253,14651647, 9657996,8157612,15175317 11553614 21515776 24386445</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:50, 2 July 2015
- Description: MCP-glutamate methylesterase / two-component response regulator-like
| Gene name | cheB |
| Synonyms | cheL |
| Essential | no |
| Product | MCP-glutamate methylesterase / two-component response regulator-like |
| Function | motility and chemotaxis |
| Gene expression levels in SubtiExpress: cheB | |
| Interactions involving this protein in SubtInteract: CheB | |
| MW, pI | 38 kDa, 8.119 |
| Gene length, protein length | 1071 bp, 357 aa |
| Immediate neighbours | flhG, cheA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
motility and chemotaxis, phosphoproteins
This gene is a member of the following regulons
CodY regulon, DegU regulon, SigD regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU16420
Phenotypes of a mutant
- not essential for pellicle biofilm formation and mutant has similar fitness to the wild-type strain when competed during pellicle formation PubMed
Database entries
- BsubCyc: BSU16420
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU16420
- Structure:
- UniProt: Q05522
- KEGG entry: [3]
- E.C. number: 3.1.1.61
Additional information
Expression and regulation
- Operon: part of the fla-che operon
- Regulation: see fla-che operon
- Regulatory mechanism: see fla-che operon
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Gerald L Hazelbauer, Wing-Cheung Lai
Bacterial chemoreceptors: providing enhanced features to two-component signaling.
Curr Opin Microbiol: 2010, 13(2);124-32
[PubMed:20122866]
[WorldCat.org]
[DOI]
(I p)
Original publications
