Difference between revisions of "PtsH"

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(Biological materials)
(Biological materials)
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** pGP961 (HPr, expression in ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], available in [[Jörg Stülke]]'s lab
 
** pGP961 (HPr, expression in ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], available in [[Jörg Stülke]]'s lab
 
** pGP1416 (HPr-H15A, expression in ''B. subtilis'', from [[pBQ200]]), available in [[Jörg Stülke]]'s lab
 
** pGP1416 (HPr-H15A, expression in ''B. subtilis'', from [[pBQ200]]), available in [[Jörg Stülke]]'s lab
** pGP2431 (N-terminal Strep-tag, purification from ''B. subtilis'', in [[pGP380]]), for [[SPINE]], available in [[Jörg Stülke]]'s lab
+
** pGP2431 (N-terminal Strep-tag, expression and purification from ''B. subtilis'', in [[pGP380]]), for [[SPINE]], available in [[Jörg Stülke]]'s lab
 
 
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 14:27, 8 December 2014

  • Description: HPr, General component of the sugar phosphotransferase system (PTS).

Gene name ptsH
Synonyms
Essential no
Product histidine-containing phosphocarrier
protein HPr of the PTS
Function PTS-dependent sugar transport
and carbon catabolite repression
Gene expression levels in SubtiExpress: ptsH
Interactions involving this protein in SubtInteract: PtsH
Metabolic function and regulation of this protein in SubtiPathways:
PtsH
MW, pI 9,1 kDa, 4.58
Gene length, protein length 264 bp, 88 amino acids
Immediate neighbours ptsG, ptsI
Sequences Protein DNA DNA_with_flanks
Genetic context
PtsH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PtsH expression.png
















Categories containing this gene/protein

phosphotransferase systems, transcription factors and their control, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

GlcT regulon, stringent response

The gene

Basic information

  • Locus tag: BSU13900

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry:[2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine (according to Swiss-Prot) Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine
  • Protein family: HPr domain (according to Swiss-Prot) HPr family
  • Paralogous protein(s): Crh

Extended information on the protein

  • Kinetic information:
  • Modification:
    • transient phosphorylation by Enzyme I of the PTS on His-15
    • regulatory phosphorylation on Ser-46 by HprK PubMed
    • an extensive study on in vivo HPr phosphorylation can be found in Singh et al. (2008) PubMed
    • weak phosphorylation on Ser-12 PubMed
    • in vitro phosphorylated by PrkC on Ser-12 PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
    • 1KKM (complex of L. casei HprK with B. subtilis HPr-Ser-P)
    • 1KKL (complex of Lactobacillus casei HprK with B. subtilis HPr)
    • 2HID (NMR)
    • 3OQM (complex of B. subtilis CcpA with P-Ser-HPr and the ackA operator site)
    • 3OQN (complex of B. subtilis CcpA with P-Ser-HPr and the gntR operator site)
    • 3OQO (complex of B. subtilis CcpA with P-Ser-HPr and a optimal synthetic operator site)
  • UniProt: P08877
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expression activated by glucose (2 fold) (GlcT) PubMed
    • the ptsH promoter is constitutive PubMed
    • subject to negative stringent control upon amino acid limitation (due to control of ptsG transcription initiation) PubMed
  • Additional information:
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 352 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 7193 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 914 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 5795 PubMed

Biological materials

  • Mutant: available in Jörg Stülke's lab:
    • MZ303 (cat)
    • GP507 ptsH1 (S46A)
    • GP506 (ptsH-H15A)
    • GP777 (replacement of glcT and the ptsG-ptsH-ptsI operon by a spc cassette), PubMed
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • CFP fusion: B. subtilis GP1267 ptsH-cfp ermC- without terminator, available in Jörg Stülke's lab
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Labs working on this gene/protein

Josef Deutscher, Paris-Grignon, France

Jörg Stülke, University of Göttingen, Germany Homepage

Wolfgang Hillen, Erlangen University, Germany Homepage

Richard Brennan, Houston, Texas, USA Homepage

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

Your additional remarks

References