YpeB

• Description: germination protein, essential for SleB assembly in spores
  
  

Gene name	ypeB
Synonyms	joeB, yzuA
Essential	no
Product	germination protein
Function	assembly of SleB
Gene expression levels in SubtiExpress: ypeB
MW, pI	51 kDa, 6.31
Gene length, protein length	1350 bp, 450 aa
Immediate neighbours	dgrA, sleB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU22920

Database entries

• BsubCyc: BSU22920

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ypeB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • has a membrane-spanning domain in the N-terminal region PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • HtrC-YpeB, for proteolysis of YpeB during germination PubMed

• Localization:
  • outer surface of the inner spore membrane PubMed

Database entries

• BsubCyc: BSU22920

• Structure:

• UniProt: P38490

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sleB-ypeB PubMed

• Expression browser: ypeB PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Casey B Bernhards, Yan Chen, Hannah Toutkoushian, David L Popham
HtrC is involved in proteolysis of YpeB during germination of Bacillus anthracis and Bacillus subtilis spores.
J Bacteriol: 2015, 197(2);326-36
[PubMed:25384476] [WorldCat.org] [DOI] (I p)

Yan Chen, W Keith Ray, Richard F Helm, Stephen B Melville, David L Popham
Levels of germination proteins in Bacillus subtilis dormant, superdormant, and germinating spores.
PLoS One: 2014, 9(4);e95781
[PubMed:24752279] [WorldCat.org] [DOI] (I e)

Yunfeng Li, Xuan Y Butzin, Andrew Davis, Barbara Setlow, George Korza, Fatma Işik Üstok, Graham Christie, Peter Setlow, Bing Hao
Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.
J Bacteriol: 2013, 195(11);2530-40
[PubMed:23543708] [WorldCat.org] [DOI] (I p)

George Korza, Peter Setlow
Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane.
J Bacteriol: 2013, 195(7);1484-91
[PubMed:23335419] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Haridasan Chirakkal, Michele O'Rourke, Abdelmadjid Atrih, Simon J Foster, Anne Moir
Analysis of spore cortex lytic enzymes and related proteins in Bacillus subtilis endospore germination.
Microbiology (Reading): 2002, 148(Pt 8);2383-2392
[PubMed:12177332] [WorldCat.org] [DOI] (P p)

Fiona M Boland, Abdelmadjid Atrih, Haridasan Chirakkal, Simon J Foster, Anne Moir
Complete spore-cortex hydrolysis during germination of Bacillus subtilis 168 requires SleB and YpeB.
Microbiology (Reading): 2000, 146 ( Pt 1);57-64
[PubMed:10658652] [WorldCat.org] [DOI] (P p)

R Moriyama, H Fukuoka, S Miyata, S Kudoh, A Hattori, S Kozuka, Y Yasuda, K Tochikubo, S Makino
Expression of a germination-specific amidase, SleB, of Bacilli in the forespore compartment of sporulating cells and its localization on the exterior side of the cortex in dormant spores.
J Bacteriol: 1999, 181(8);2373-8
[PubMed:10197998] [WorldCat.org] [DOI] (P p)