SleB

• Description: spore cortex-lytic enzyme
  
  

Gene name	sleB
Synonyms	ypeA
Essential	no
Product	spore cortex-lytic enzyme
Function	degration of the spore cortex, germination
Gene expression levels in SubtiExpress: sleB
MW, pI	33 kDa, 9.506
Gene length, protein length	915 bp, 305 aa
Immediate neighbours	ypeB, prsW
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU22930

Database entries

• BsubCyc: BSU22930

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sleB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • contains a signal peptide PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • outer surface of the inner spore membrane PubMed

Database entries

• BsubCyc: BSU22930

• Structure:

• UniProt: P50739

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sleB-ypeB PubMed

• Expression browser: sleB PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1G21 ( sleB::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

B Setlow, J Yu, Y-Q Li, P Setlow
Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite.
Lett Appl Microbiol: 2013, 57(4);259-65
[PubMed:23746146] [WorldCat.org] [DOI] (I p)

Yunfeng Li, Xuan Y Butzin, Andrew Davis, Barbara Setlow, George Korza, Fatma Işik Üstok, Graham Christie, Peter Setlow, Bing Hao
Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.
J Bacteriol: 2013, 195(11);2530-40
[PubMed:23543708] [WorldCat.org] [DOI] (I p)

George Korza, Peter Setlow
Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane.
J Bacteriol: 2013, 195(7);1484-91
[PubMed:23335419] [WorldCat.org] [DOI] (I p)

Xuan Yi, Peter Setlow
Studies of the commitment step in the germination of spores of bacillus species.
J Bacteriol: 2010, 192(13);3424-33
[PubMed:20435722] [WorldCat.org] [DOI] (I p)

Atsushi Masayama, Hideyuki Fukuoka, Shiro Kato, Tohru Yoshimura, Michie Moriyama, Ryuichi Moriyama
Subcellular localization of a germiantion-specific cortex-lytic enzyme, SleB, of Bacilli during sporulation.
Genes Genet Syst: 2006, 81(3);163-9
[PubMed:16905870] [WorldCat.org] [DOI] (P p)

Barbara Setlow, Swaroopa Atluri, Ryan Kitchel, Kasia Koziol-Dube, Peter Setlow
Role of dipicolinic acid in resistance and stability of spores of Bacillus subtilis with or without DNA-protective alpha/beta-type small acid-soluble proteins.
J Bacteriol: 2006, 188(11);3740-7
[PubMed:16707666] [WorldCat.org] [DOI] (P p)

Haridasan Chirakkal, Michele O'Rourke, Abdelmadjid Atrih, Simon J Foster, Anne Moir
Analysis of spore cortex lytic enzymes and related proteins in Bacillus subtilis endospore germination.
Microbiology (Reading): 2002, 148(Pt 8);2383-2392
[PubMed:12177332] [WorldCat.org] [DOI] (P p)

Fiona M Boland, Abdelmadjid Atrih, Haridasan Chirakkal, Simon J Foster, Anne Moir
Complete spore-cortex hydrolysis during germination of Bacillus subtilis 168 requires SleB and YpeB.
Microbiology (Reading): 2000, 146 ( Pt 1);57-64
[PubMed:10658652] [WorldCat.org] [DOI] (P p)

R Moriyama, H Fukuoka, S Miyata, S Kudoh, A Hattori, S Kozuka, Y Yasuda, K Tochikubo, S Makino
Expression of a germination-specific amidase, SleB, of Bacilli in the forespore compartment of sporulating cells and its localization on the exterior side of the cortex in dormant spores.
J Bacteriol: 1999, 181(8);2373-8
[PubMed:10197998] [WorldCat.org] [DOI] (P p)