Difference between revisions of "FrlO"

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** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
 
** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
 
** the ''[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' operon is not expressed in a ''[[cshA]]'' mutant {{PubMed|23175651}}
 
** the ''[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' operon is not expressed in a ''[[cshA]]'' mutant {{PubMed|23175651}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 696 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 11:25, 17 April 2014

Gene name frlO
Synonyms yurO
Essential no
Product aminosugar ABC transporter (binding protein)
Function uptake of sugar amines
Gene expression levels in SubtiExpress: frlO
Interactions involving this protein in SubtInteract: FrlO
Metabolic function and regulation of this protein in SubtiPathways:
frlO
MW, pI 47 kDa, 5.674
Gene length, protein length 1266 bp, 422 aa
Immediate neighbours frlN, frlB
Sequences Protein DNA DNA_with_flanks
Genetic context
YurO context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FrlO expression.png















Categories containing this gene/protein

ABC transporters, utilization of specific carbon sources, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon, FrlR regulon

The gene

Basic information

  • Locus tag: BSU32600

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: bacterial solute-binding protein 1 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • frlB: repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • frlB: induced in the presence of fructosamine (FrlR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References