Dat

• Description: D-alanine aminotransferase
  
  

Gene name	dat
Synonyms	yheM
Essential	no
Product	D-alanine aminotransferase
Function	peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: dat
Metabolic function and regulation of this protein in SubtiPathways: dat
MW, pI	31 kDa, 4.871
Gene length, protein length	846 bp, 282 aa
Immediate neighbours	yheN, nhaC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components

The gene

Basic information

• Locus tag: BSU09670

Database entries

• BsubCyc: BSU09670

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• Another gene coding for O6-methylguanine DNA alkyltransferase was formely known as dat, it is now called ogt

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-alanine + 2-oxoglutarate = pyruvate + D-glutamate (according to Swiss-Prot)

• Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU09670

• Structure: 1G2W (E177S Mutant, Geobacillus stearothermophilus, 43% identity)

• UniProt: O07597

• KEGG entry: [2]

• E.C. number: 2.6.1.21

Expression and regulation

• Operon:

• Expression browser: dat PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1262 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 794 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: