Difference between revisions of "YfmL"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU07430&redirect=T BSU07430] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfmL.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfmL.html] | ||
| Line 89: | Line 90: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU07430&redirect=T BSU07430] | ||
* '''Structure:''' | * '''Structure:''' | ||
Revision as of 13:11, 2 April 2014
- Description: DEAD-box RNA helicase
| Gene name | yfmL |
| Synonyms | |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| Gene expression levels in SubtiExpress: yfmL | |
| MW, pI | 42 kDa, 9.602 |
| Gene length, protein length | 1128 bp, 376 aa |
| Immediate neighbours | yfmM, yfmK |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DEAD-box RNA helicases, translation
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU07430
Phenotypes of a mutant
- poor growth at low temperatures (16 to 20°C) PubMed
Database entries
- BsubCyc: BSU07430
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: RNA helicase
- Protein family: helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicase
- Paralogous protein(s): CshA, CshB, DeaD
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU07430
- Structure:
- UniProt: O34750
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: yfmL (according to DBTBS)
- Regulation:
- expression declines in the stationary phase PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1053 (erm), available in the Stülke lab
- Expression vector:
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1069 (spc), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- B. subtilis GP1082 yfmL-yfp spc, available in Jörg Stülke's lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1066 (spc, based on pGP1331), available in Jörg Stülke's
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
