Difference between revisions of "GerD"

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* '''Description:''' required for clustering of germinant receptors in the spore inner membrane<br/><br/>
+
* '''Description:''' scaffold of the [[germinosome]], required for clustering of germinant receptors in the spore inner membrane<br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* defective in [[germination]] in response to nutrients {{PubMed|24530795}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
 
  
 
=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
 
** has an N-terminal signal sequence and a signal for fixation to lipid {{PubMed|23335419}}
 
** has an N-terminal signal sequence and a signal for fixation to lipid {{PubMed|23335419}}
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
 
* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
 +
** forms trimeric rope-like structures {{PubMed|24530795}}
 
** [[GerD]] is part of the [[germinosome]] in the spore inner membrane {{PubMed|21696470}}
 
** [[GerD]] is part of the [[germinosome]] in the spore inner membrane {{PubMed|21696470}}
 
** [[GerD]]-[[Lgt]] to fix [[GerD]] to diacylglycerol in the membrane {{PubMed|22493018}}
 
** [[GerD]]-[[Lgt]] to fix [[GerD]] to diacylglycerol in the membrane {{PubMed|22493018}}
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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
+
* '''Structure:''' [http://pdb.org/pdb/explore/explore.do?structureId=4O8W 4O8W] {{PubMed|24530795}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P16450 P16450]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P16450 P16450]
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gerD_158515_159072_-1 gerD] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gerD_158515_159072_-1 gerD] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigF]] {{PubMed|15699190}}, [[SigG]] {{PubMed|1906867,15699190}}
+
* '''[[Sigma factor]]:''' [[SigF]] {{PubMed|15699190}}, [[SigG]] {{PubMed|1906867,15699190}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
<pubmed>1906867,19332816,17122337, 21725007,18556788, 19332816 2517635 21696470 22327596 22493018 23335419 23749970 21398556</pubmed>
+
<pubmed>1906867,19332816,17122337, 21725007,18556788, 19332816 2517635 21696470 22327596 22493018 23335419 23749970 21398556 24530795 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 18:29, 18 February 2014

  • Description: scaffold of the germinosome, required for clustering of germinant receptors in the spore inner membrane

Gene name gerD
Synonyms
Essential no
Product lipoprotein
Function germination
Gene expression levels in SubtiExpress: gerD
Interactions involving this protein in SubtInteract: GerD
MW, pI 20 kDa, 4.862
Gene length, protein length 555 bp, 185 aa
Immediate neighbours salA, kbaA
Sequences Protein DNA DNA_with_flanks
Genetic context
GerD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GerD expression.png















Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon

The gene

Basic information

  • Locus tag: BSU01550

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: required for clustering of germinant receptors in the spore inner membrane PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • has an N-terminal signal sequence and a signal for fixation to lipid PubMed
  • Modification:
  • Effectors of protein activity:
  • Localization:
    • outer surface of the inner spore membrane PubMed
    • inner spore membrane, spore coat, cortex, germ cell wall, some GerD is also present in the soluble fraction PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

  • 3,500 molecules are present per spore PubMed

Expression and regulation

  • Regulation:
    • expression level depends on sporulation conditions (medim composition, temperature) PubMed
  • Regulatory mechanism:
  • Additional information:
    • 3,500 molecules are present per spore PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:

Labs working on this gene/protein

Anne Moir, University of Sheffield, UK

Your additional remarks

References