Difference between revisions of "DapI"
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of lysine and peptidoglycan | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of lysine and peptidoglycan | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14190 | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14190 dapI] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/lys_threo.html Lys, Thr]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/lys_threo.html Lys, Thr]''' | ||
| Line 71: | Line 71: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' N-acetyl-LL-2,6- | + | * '''Catalyzed reaction/ biological activity:''' N-acetyl-LL-2,6-diaminopimelate + H<sub>2</sub>O --> acetate + LL-2,6-diaminopimelate {{PubMed|24261876}} |
* '''Protein family:''' peptidase M20A family (according to Swiss-Prot) | * '''Protein family:''' peptidase M20A family (according to Swiss-Prot) | ||
| Line 81: | Line 81: | ||
* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** [[DapI]]-[[MreB]] {{PubMed|24261876}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| + | ** forms uniformly distributed foci in the cytoplasm {{PubMed|24261876}} | ||
=== Database entries === | === Database entries === | ||
| Line 118: | Line 120: | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| − | |||
=Biological materials = | =Biological materials = | ||
| Line 139: | Line 140: | ||
=References= | =References= | ||
| − | + | <pubmed>24261876 </pubmed> | |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:01, 5 January 2014
- Description: N-acetyl-diaminopimelate deacetylase
| Gene name | ykuR |
| Synonyms | dapL |
| Essential | yes PubMed |
| Product | N-acetyl-diaminopimelate deacetylase |
| Function | biosynthesis of lysine and peptidoglycan |
| Gene expression levels in SubtiExpress: dapI | |
| Metabolic function and regulation of this protein in SubtiPathways: Lys, Thr | |
| MW, pI | 41 kDa, 5.102 |
| Gene length, protein length | 1122 bp, 374 aa |
| Immediate neighbours | ykuQ, ykuS |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall synthesis, biosynthesis of cell wall components, biosynthesis/ acquisition of amino acids, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU14190
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: N-acetyl-LL-2,6-diaminopimelate + H2O --> acetate + LL-2,6-diaminopimelate PubMed
- Protein family: peptidase M20A family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization:
- forms uniformly distributed foci in the cytoplasm PubMed
Database entries
- Structure:
- UniProt: O34916
- KEGG entry: [2]
- E.C. number: 3.5.1.47
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Anne-Stéphanie Rueff, Arnaud Chastanet, Julia Domínguez-Escobar, Zhizhong Yao, James Yates, Maria-Victoria Prejean, Olivier Delumeau, Philippe Noirot, Roland Wedlich-Söldner, Sergio R Filipe, Rut Carballido-López
An early cytoplasmic step of peptidoglycan synthesis is associated to MreB in Bacillus subtilis.
Mol Microbiol: 2014, 91(2);348-62
[PubMed:24261876]
[WorldCat.org]
[DOI]
(I p)
