DapI

• Description: N-acetyl-diaminopimelate deacetylase
  
  

Gene name	ykuR
Synonyms	dapL
Essential	yes PubMed
Product	N-acetyl-diaminopimelate deacetylase
Function	biosynthesis of lysine and peptidoglycan
Gene expression levels in SubtiExpress: dapI
Interactions involving this protein in SubtInteract: DapI
Metabolic function and regulation of this protein in SubtiPathways: DapI
MW, pI	41 kDa, 5.102
Gene length, protein length	1122 bp, 374 aa
Immediate neighbours	ykuQ, ykuS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, biosynthesis/ acquisition of amino acids, essential genes

The gene

Basic information

• Locus tag: BSU14190

Phenotypes of a mutant

• essential PubMed
• depletion of DapI results in swelling and rounding up of cells prior to lysis PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: N-acetyl-LL-2,6-diaminopimelate + H₂O --> acetate + LL-2,6-diaminopimelate PubMed

• Protein family: peptidase M20A family (according to Swiss-Prot)

• Paralogous protein(s): SndA, SndB, SndC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed
  • DapI-MreB PubMed

• Localization:
  • forms uniformly distributed foci in the cytoplasm PubMed

Database entries

• Structure:

• UniProt: O34916

• KEGG entry: [2]

• E.C. number: 3.5.1.47

Expression and regulation

• Operon:

• Expression browser: dapI PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 274 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 637 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Anne-Stéphanie Rueff, Arnaud Chastanet, Julia Domínguez-Escobar, Zhizhong Yao, James Yates, Maria-Victoria Prejean, Olivier Delumeau, Philippe Noirot, Roland Wedlich-Söldner, Sergio R Filipe, Rut Carballido-López
An early cytoplasmic step of peptidoglycan synthesis is associated to MreB in Bacillus subtilis.
Mol Microbiol: 2014, 91(2);348-62
[PubMed:24261876] [WorldCat.org] [DOI] (I p)