Difference between revisions of "SpoIIIE"

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(Original publications)
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** translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes {{PubMed|19818024}}
 
** translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes {{PubMed|19818024}}
 
** the two DNA translocases [[SftA]] and [[SpoIIIE]] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively {{PubMed|21239579}}
 
** the two DNA translocases [[SftA]] and [[SpoIIIE]] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively {{PubMed|21239579}}
 +
** binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG {{PubMed|18391964}}
  
 
* '''Protein family:'''
 
* '''Protein family:'''
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
 +
** N-terminal transmembrane domain (4 helices) {{PubMed|24297254}}
 +
** 134 aa linker {{PubMed|24297254}}
 +
** 512 aa motor domain at the C-terminus {{PubMed|24297254}}
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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<pubmed> 22047950 22934648 23400100 </pubmed>
 
<pubmed> 22047950 22934648 23400100 </pubmed>
 
==Original publications==
 
==Original publications==
<pubmed> 18160039, 18593879, 3129532 2507870 18593879 23559069 9150232,2492502, 11062134,9155041, 14680637,7797072, 7567988, 2507870,8160014,9515703, 19818024 19788545 20298190 20516200 21239579 18391964 23667326 11134515 </pubmed>
+
<pubmed> 18160039, 18593879, 3129532 2507870 18593879 23559069 9150232,2492502, 11062134,9155041, 14680637,7797072, 7567988, 2507870,8160014,9515703, 19818024 19788545 20298190 20516200 21239579 18391964 23667326 11134515 24297254</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 18:38, 4 December 2013

  • Description: ATP-dependent DNA translocase, resolution of chromosomal dimers after DNA replication, transports the forespore chromosome across the sporulation septum

Gene name spoIIIE
Synonyms
Essential no
Product ATP-dependent DNA translocase required for chromosome

partitioning through the septum into the forespore

Function chromosome partition during sporulation
Gene expression levels in SubtiExpress: spoIIIE
MW, pI 86 kDa, 6.757
Gene length, protein length 2361 bp, 787 aa
Immediate neighbours ylzJ, ymfC
Sequences Protein DNA DNA_with_flanks
Genetic context
SpoIIIE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpoIIIE expression.png















Categories containing this gene/protein

DNA condensation/ segregation, sporulation/ other, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU16800

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • transports the forespore chromosome across the sporulation septum PubMed
    • translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes PubMed
    • the two DNA translocases SftA and SpoIIIE synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively PubMed
    • binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG PubMed
  • Protein family:
  • Paralogous protein(s): SftA

Extended information on the protein

  • Kinetic information:
  • Domains:
    • N-terminal transmembrane domain (4 helices) PubMed
    • 134 aa linker PubMed
    • 512 aa motor domain at the C-terminus PubMed
  • Modification:
  • Effectors of protein activity:
  • Localization:
    • transmembrane domain mediates dynamic localization to active division sites PubMed
    • complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of sporulation and division septa PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications