SipW

• Description: bifunctional signal peptidase I that controls surface-adhered biofilm formation and processes TasA and TapA
  
  

Gene name	sipW
Synonyms	yqhE
Essential	no
Product	signal peptidase I
Function	biofilm formation
Gene expression levels in SubtiExpress: sipW
Interactions involving this protein in SubtInteract: SipW
Regulation of this protein in SubtiPathways: Biofilm, Protein secretion
MW, pI	20 kDa, 5.494
Gene length, protein length	570 bp, 190 aa
Immediate neighbours	tasA, tapA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein secretion, biofilm formation, membrane proteins

This gene is a member of the following regulons

AbrB regulon, RemA regulon, SinR regulon

The gene

Basic information

• Locus tag: BSU24630

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • important for biofilm formation on a solid surface, but not required at an air-liquid interface PubMed
  • Cleavage of hydrophobic, N-terminal signal or leader sequences from TasA and TapA PubMed

• Protein family: peptidase S26B family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SipW-TapA PubMed
  • SipW-TasA PubMed

• Localization:
  • membrane PubMed

Database entries

• Structure:

• UniProt: P54506

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tapA-sipW-tasA PubMed

• Expression browser: sipW PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by SinR PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • SinR: transcription repression PubMed
  • RemA: transcription activation PubMed

• Additional information:
  • induction by sequestration of SinR by SinI or SlrA PubMed or by SlrR PubMed
  • the tapA-sipW-tasA operon is not expressed in a ymdB mutant PubMed
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed

Biological materials

• Mutant:
  • 1A937 ( sipW::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Beth Lazazzera, Los Angeles, USA