Difference between revisions of "SplB"

From SubtiWiki
Jump to: navigation, search
(Database entries)
Line 91: Line 91:
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' http://www.rcsb.org/pdb/explore/explore.do?structureId=4FHC 4FHC (SplB from ''Geobacillus thermodenitrificans'', 72% identity, 90% similarity) {{PubMed|22761404}}
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4FHC 4FHC] (SplB from ''Geobacillus thermodenitrificans'', 72% identity, 90% similarity) {{PubMed|22761404}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37956 P37956]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37956 P37956]

Revision as of 14:32, 15 August 2013

  • Description: spore photoproduct lyase

Gene name splB
Synonyms spl
Essential no
Product spore photoproduct lyase
Function protection of spore DNA against photodamage
Gene expression levels in SubtiExpress: splB
MW, pI 39 kDa, 8.524
Gene length, protein length 1026 bp, 342 aa
Immediate neighbours splA, ykwB
Sequences Protein DNA DNA_with_flanks
Genetic context
SplB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SplB expression.png















Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

  • Locus tag: BSU13930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
    • contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAM
  • Effectors of protein activity:

Database entries

  • Structure: 4FHC (SplB from Geobacillus thermodenitrificans, 72% identity, 90% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Alhosna Benjdia
DNA photolyases and SP lyase: structure and mechanism of light-dependent and independent DNA lyases.
Curr Opin Struct Biol: 2012, 22(6);711-20
[PubMed:23164663] [WorldCat.org] [DOI] (I p)

Original publications