Difference between revisions of "Gpr"
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| − | * '''Description:''' | + | * '''Description:''' [[germination]] protease (degradation of SASPs) <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || [[germination]] protease (degradation of SASPs) |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || degradation of SASPs |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25540 gpr] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25540 gpr] | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
| − | + | <br/><br/> | |
| − | |||
| − | |||
| − | |||
| − | |||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[proteolysis]]}}, | ||
{{SubtiWiki category|[[germination]]}} | {{SubtiWiki category|[[germination]]}} | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
{{SubtiWiki regulon|[[SigF regulon]]}}, | {{SubtiWiki regulon|[[SigF regulon]]}}, | ||
| − | {{SubtiWiki regulon|[[SigG regulon]]}} | + | {{SubtiWiki regulon|[[SigG regulon]]}}, |
| + | {{SubtiWiki regulon|[[SpoVT regulon]]}} | ||
=The gene= | =The gene= | ||
| Line 65: | Line 63: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| Line 73: | Line 69: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' degradation of small, acid-soluble spore proteins (SASPs) | + | * '''Catalyzed reaction/ biological activity:''' degradation of small, acid-soluble spore proteins (SASPs) {{PubMed|8478323}} |
* '''Protein family:''' peptidase A25 family (according to Swiss-Prot) | * '''Protein family:''' peptidase A25 family (according to Swiss-Prot) | ||
| Line 115: | Line 111: | ||
* '''Sigma factor:''' [[SigF]] {{PubMed|16497325,1840582}}, [[SigG]] {{PubMed|1840582}} | * '''Sigma factor:''' [[SigF]] {{PubMed|16497325,1840582}}, [[SigG]] {{PubMed|1840582}} | ||
| − | * '''Regulation:''' expressed during sporulation in the forespore ([[SigF]], [[SigG]]) {{PubMed|16497325,1840582}} | + | * '''Regulation:''' expressed during sporulation in the forespore ([[SigF]], [[SigG]], [[SpoVT]]) {{PubMed|16497325,1840582}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[SpoVT]]: transcription repression {{PubMed|16497325}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 141: | Line 138: | ||
=References= | =References= | ||
| − | <pubmed>16199582,9045848,8188581,8071242,1732215,16497325,7961468,8071212,9748439,1840582,7608092,8478323, </pubmed> | + | <pubmed>16199582,9045848,8188581,8071242,1732215,16497325,7961468,8071212,9748439,1840582,7608092,8478323, 23927687 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:50, 12 August 2013
- Description: germination protease (degradation of SASPs)
| Gene name | gpr |
| Synonyms | |
| Essential | no |
| Product | germination protease (degradation of SASPs) |
| Function | degradation of SASPs |
| Gene expression levels in SubtiExpress: gpr | |
| MW, pI | 40 kDa, 5.199 |
| Gene length, protein length | 1104 bp, 368 aa |
| Immediate neighbours | spoIIP, rpsT |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
SigF regulon, SigG regulon, SpoVT regulon
The gene
Basic information
- Locus tag: BSU25540
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: degradation of small, acid-soluble spore proteins (SASPs) PubMed
- Protein family: peptidase A25 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P22322
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Bjorn A Traag, Antonia Pugliese, Barbara Setlow, Peter Setlow, Richard Losick
A conserved ClpP-like protease involved in spore outgrowth in Bacillus subtilis.
Mol Microbiol: 2013, 90(1);160-6
[PubMed:23927687]
[WorldCat.org]
[DOI]
(I p)
Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325]
[WorldCat.org]
[DOI]
(P p)
Thomas M Carroll, Peter Setlow
Site-directed mutagenesis and structural studies suggest that the germination protease, GPR, in spores of Bacillus species is an atypical aspartic acid protease.
J Bacteriol: 2005, 187(20);7119-25
[PubMed:16199582]
[WorldCat.org]
[DOI]
(P p)
C Nessi, M J Jedrzejas, P Setlow
Structure and mechanism of action of the protease that degrades small, acid-soluble spore proteins during germination of spores of Bacillus species.
J Bacteriol: 1998, 180(19);5077-84
[PubMed:9748439]
[WorldCat.org]
[DOI]
(P p)
L B Pedersen, C Nessi, P Setlow
Most of the propeptide is dispensable for stability and autoprocessing of the zymogen of the germination protease of spores of Bacillus species.
J Bacteriol: 1997, 179(5);1824-7
[PubMed:9045848]
[WorldCat.org]
[DOI]
(P p)
B Setlow, P Setlow
Binding to DNA protects alpha/beta-type, small, acid-soluble spore proteins of Bacillus and Clostridium species against digestion by their specific protease as well as by other proteases.
J Bacteriol: 1995, 177(14);4149-51
[PubMed:7608092]
[WorldCat.org]
[DOI]
(P p)
B Illades-Aguiar, P Setlow
Autoprocessing of the protease that degrades small, acid-soluble proteins of spores of Bacillus species is triggered by low pH, dehydration, and dipicolinic acid.
J Bacteriol: 1994, 176(22);7032-7
[PubMed:7961468]
[WorldCat.org]
[DOI]
(P p)
B Illades-Aguiar, P Setlow
The zymogen of the protease that degrades small, acid-soluble proteins of spores of Bacillus species can rapidly autoprocess to the active enzyme in vitro.
J Bacteriol: 1994, 176(17);5571-3
[PubMed:8071242]
[WorldCat.org]
[DOI]
(P p)
Y Carrillo-Martinez, P Setlow
Properties of Bacillus subtilis small, acid-soluble spore proteins with changes in the sequence recognized by their specific protease.
J Bacteriol: 1994, 176(17);5357-63
[PubMed:8071212]
[WorldCat.org]
[DOI]
(P p)
B Illades-Aguiar, P Setlow
Studies of the processing of the protease which initiates degradation of small, acid-soluble proteins during germination of spores of Bacillus species.
J Bacteriol: 1994, 176(10);2788-95
[PubMed:8188581]
[WorldCat.org]
[DOI]
(P p)
J L Sanchez-Salas, P Setlow
Proteolytic processing of the protease which initiates degradation of small, acid-soluble proteins during germination of Bacillus subtilis spores.
J Bacteriol: 1993, 175(9);2568-77
[PubMed:8478323]
[WorldCat.org]
[DOI]
(P p)
J L Sanchez-Salas, M L Santiago-Lara, B Setlow, M D Sussman, P Setlow
Properties of Bacillus megaterium and Bacillus subtilis mutants which lack the protease that degrades small, acid-soluble proteins during spore germination.
J Bacteriol: 1992, 174(3);807-14
[PubMed:1732215]
[WorldCat.org]
[DOI]
(P p)
M D Sussman, P Setlow
Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination.
J Bacteriol: 1991, 173(1);291-300
[PubMed:1840582]
[WorldCat.org]
[DOI]
(P p)
