Difference between revisions of "DnaN"
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| − | * '''Description:''' DNA polymerase III (beta subunit), beta clamp, part of the [[replisome]], also involved in DNA mismatch repair <br/><br/> | + | * '''Description:''' DNA polymerase III (beta subunit), beta clamp, part of the [[replisome]], also involved in DNA mismatch repair, inhibitor of [[DnaA]] oligomerization <br/><br/> |
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** recruits [[MutS]] to the site of [[DNA replication]] ([[replisome]]) {{PubMed|23228104}} | ** recruits [[MutS]] to the site of [[DNA replication]] ([[replisome]]) {{PubMed|23228104}} | ||
** required for bacteriophage SPP1 replication {{PubMed|23268446}} | ** required for bacteriophage SPP1 replication {{PubMed|23268446}} | ||
| − | ** inhibits oligomerization of [[DnaA]] {{PubMed|23909787}} | + | ** inhibits oligomerization and helix formation of [[DnaA]] {{PubMed|23909787}} |
* '''Protein family:''' | * '''Protein family:''' | ||
Revision as of 16:46, 8 August 2013
- Description: DNA polymerase III (beta subunit), beta clamp, part of the replisome, also involved in DNA mismatch repair, inhibitor of DnaA oligomerization
| Gene name | dnaN |
| Synonyms | dnaG, dnaK |
| Essential | yes PubMed |
| Product | DNA polymerase III (beta subunit), beta clamp |
| Function | DNA replication, DNA repair |
| Gene expression levels in SubtiExpress: DnaN | |
| Interactions involving this protein in SubtInteract: DnaN | |
| MW, pI | 41 kDa, 4.718 |
| Gene length, protein length | 1134 bp, 378 aa |
| Immediate neighbours | dnaA, yaaA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA replication, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00020
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- DnaN overexpression or release from the replisome decreases association of YabA with oriC, increases association of DnaA with oriC PubMed
- Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
- recruits MutS to the site of DNA replication (replisome) PubMed
- required for bacteriophage SPP1 replication PubMed
- inhibits oligomerization and helix formation of DnaA PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- DnaA-YabA-DnaN PubMed
- part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
- PolY1-DnaN, PolY2-DnaN (required for untargeted mutagenesis) PubMed
- PolY1-PolA-DnaN PubMed
- MutL (C-terminal domain)-DnaN PubMed
- MutS-DnaN PubMed
- DnaE-DnaN PubMed
- DnaN forms dimers PubMed
Database entries
- Structure:
- UniProt: P05649
- KEGG entry: [3]
- E.C. number: 2.7.7.7
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Philippe Noirot, Jouy-en-Josas, France homepage
Your additional remarks
References
Reviews
Tsutomu Katayama, Shogo Ozaki, Kenji Keyamura, Kazuyuki Fujimitsu
Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.
Nat Rev Microbiol: 2010, 8(3);163-70
[PubMed:20157337]
[WorldCat.org]
[DOI]
(I p)
Original publications
