Difference between revisions of "YkoV"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13410 ykoV]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13410 ykoV]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/YkoV YkoV]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 7.213   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 7.213   
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
+
* sensitivity to ionizing radiation in the stationary phase  {{PubMed|12215643}}
 +
* sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat {{PubMed|16497325,17293412}}
 
=== Database entries ===
 
=== Database entries ===
  
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 +
** [[YkoV]]-[[LigD]] {{PubMed|23691176}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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<pubmed> 11591342 11719239 17938628 </pubmed>
 
<pubmed> 11591342 11719239 17938628 </pubmed>
 
== Original publications ==
 
== Original publications ==
<pubmed>16497325, 11483577, 23691176 12215643 </pubmed>
+
<pubmed>16497325, 11483577, 23691176 12215643 17293412</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:33, 23 May 2013

  • Description: DNA-end-binding protein Ku, recruits LigD to DNA ends, confers dry-heat resistance to dormant spores PubMed



Gene name ykoV
Synonyms
Essential no
Product DNA-end-binding protein Ku
Function non-homologous end joining DNA repair
Gene expression levels in SubtiExpress: ykoV
Interactions involving this protein in SubtInteract: YkoV
MW, pI 34 kDa, 7.213
Gene length, protein length 933 bp, 311 aa
Immediate neighbours ligD, ykoW
Sequences Protein DNA DNA_with_flanks
Genetic context
YkoV context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkoV expression.png















Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

  • Locus tag: BSU13410

Phenotypes of a mutant

  • sensitivity to ionizing radiation in the stationary phase PubMed
  • sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed during sporulation in the forespore (SigG, SpoVT) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Miguel de Vega
The minimal Bacillus subtilis nonhomologous end joining repair machinery.
PLoS One: 2013, 8(5);e64232
[PubMed:23691176] [WorldCat.org] [DOI] (I e)

Ralf Moeller, Erko Stackebrandt, Günther Reitz, Thomas Berger, Petra Rettberg, Aidan J Doherty, Gerda Horneck, Wayne L Nicholson
Role of DNA repair by nonhomologous-end joining in Bacillus subtilis spore resistance to extreme dryness, mono- and polychromatic UV, and ionizing radiation.
J Bacteriol: 2007, 189(8);3306-11
[PubMed:17293412] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Geoffrey R Weller, Boris Kysela, Rajat Roy, Louise M Tonkin, Elizabeth Scanlan, Marina Della, Susanne Krogh Devine, Jonathan P Day, Adam Wilkinson, Fabrizio d'Adda di Fagagna, Kevin M Devine, Richard P Bowater, Penny A Jeggo, Stephen P Jackson, Aidan J Doherty
Identification of a DNA nonhomologous end-joining complex in bacteria.
Science: 2002, 297(5587);1686-9
[PubMed:12215643] [WorldCat.org] [DOI] (I p)

L Aravind, E V Koonin
Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system.
Genome Res: 2001, 11(8);1365-74
[PubMed:11483577] [WorldCat.org] [DOI] (P p)