ProB

• Description: glutamate 5-kinase
  
  

Gene name	proB
Synonyms
Essential	no
Product	glutamate 5-kinase
Function	biosynthesis of proline
Gene expression levels in SubtiExpress: proB
Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress
MW, pI	39 kDa, 6.01
Gene length, protein length	1095 bp, 365 aa
Immediate neighbours	ykkE, proA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU13120

Phenotypes of a mutant

• auxotrophic for proline PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)

• Protein family: PUA domain (according to Swiss-Prot)

• Paralogous protein(s): ProJ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: the enzymatic activity is feedback-inhibited by proline {PubMed|16958849}}

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39820

• KEGG entry: [2]

• E.C. number: 2.7.2.11

Expression and regulation

• Operon: proB-proAPubMed

• Expression browser: proB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by proline limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed