RsiW

• Description: anti-SigW
  
  

Gene name	rsiW
Synonyms	ybbM
Essential	no
Product	anti-SigW
Function	control of SigW activity
Gene expression levels in SubtiExpress: rsiW
Interactions involving this protein in SubtInteract: RsiW
MW, pI	23 kDa, 6.646
Gene length, protein length	624 bp, 208 aa
Immediate neighbours	sigW, cdaA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

AbrB regulon, SigW regulon

The gene

Basic information

• Locus tag: BSU01740

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: anti-sigma-W factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: degraded by RasP and PrsW under conditions of alkali shock or in the presence of antimicrobial peptides, respectively. This results in the release of SigW

• Interactions:
  • PrsW-RsiW (proteolysis) PubMed
  • RasP-RsiW (proteolysis)
  • RsiW-SigW PubMed

• Localization: membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q45588

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sigW-rsiW PubMed

• Expression browser: rsiW PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • induced under conditions of alkali shock or in the presence of the toxin SdpC (SigW) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Thomas Wiegert, University of Bayreuth, Germany Homepage

References

Reviews

Additional reviews: PubMed

Gu Chen, Xu Zhang
New insights into S2P signaling cascades: regulation, variation, and conservation.
Protein Sci: 2010, 19(11);2015-30
[PubMed:20836086] [WorldCat.org] [DOI] (I p)