Difference between revisions of "ExoA"

From SubtiWiki
Jump to: navigation, search
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || repair of oxidative DNA damage in spores
 
|style="background:#ABCDEF;" align="center"|'''Function''' || repair of oxidative DNA damage in spores
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU40880 exoA]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40880 exoA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 4.592   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 4.592   
Line 22: Line 22:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ccpB]]'', ''[[rpsR]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ccpB]]'', ''[[rpsR]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB16125&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40880 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40880 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40880 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:exoA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:exoA_context.gif]]

Revision as of 14:20, 13 May 2013

  • Description: apurinic/apyrimidinic endonuclease, multifunctional DNA-repair enzyme, important for spore dormance

Gene name exoA
Synonyms
Essential no
Product apurinic/apyrimidinic endonuclease
Function repair of oxidative DNA damage in spores
Gene expression levels in SubtiExpress: exoA
MW, pI 29 kDa, 4.592
Gene length, protein length 756 bp, 252 aa
Immediate neighbours ccpB, rpsR
Sequences Protein DNA Advanced_DNA
Genetic context
ExoA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ExoA expression.png
























Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

  • Locus tag: BSU40880

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • ExoA is functionally redundant with Nfo

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (according to Swiss-Prot)
  • Protein family: DNA repair enzymes AP/exoA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed