Difference between revisions of "PolY2"

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|style="background:#ABCDEF;" align="center"|'''Function''' || UV-targeted mutagenesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || UV-targeted mutagenesis
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU23710 polY2]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23710 polY2]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 9.532   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 9.532   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqjX]]'', ''[[yqzH]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqjX]]'', ''[[yqzH]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14303&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23710 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23710 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23710 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yqjW_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yqjW_context.gif]]

Revision as of 13:13, 13 May 2013

  • Description: translesion synthesis (TLS-) DNA polymerase Y2

Gene name polY2
Synonyms yqjW
Essential no
Product translesion synthesis (TLS-) DNA polymerase Y2
Function UV-targeted mutagenesis
Gene expression levels in SubtiExpress: polY2
MW, pI 45 kDa, 9.532
Gene length, protein length 1236 bp, 412 aa
Immediate neighbours yqjX, yqzH
Sequences Protein DNA Advanced_DNA
Genetic context
YqjW context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PolY2 expression.png
























Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

  • Locus tag: BSU23710

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • Protein family: DNA polymerase type-Y family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1505 (polY2::kan), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References