Difference between revisions of "SspB"
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|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA | |style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09750 sspB] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 4.791 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 4.791 | ||
| Line 21: | Line 21: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yheF]]'', ''[[yheE]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yheF]]'', ''[[yheE]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU09750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU09750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU09750 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:sspB_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:sspB_context.gif]] | ||
Revision as of 12:27, 13 May 2013
- Description: small acid-soluble spore protein (major beta-type SASP)
| Gene name | sspB |
| Synonyms | |
| Essential | no |
| Product | small acid-soluble spore protein (major beta-type SASP) |
| Function | protection of spore DNA |
| Gene expression levels in SubtiExpress: sspB | |
| MW, pI | 6 kDa, 4.791 |
| Gene length, protein length | 201 bp, 67 aa |
| Immediate neighbours | yheF, yheE |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU09750
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: alpha/beta-type SASP family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P04832
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: sspB PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion: GP996 (cat, based on pSG1151), available in the Stülke lab
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ralf Moeller, Andrew C Schuerger, Günther Reitz, Wayne L Nicholson
Protective role of spore structural components in determining Bacillus subtilis spore resistance to simulated mars surface conditions.
Appl Environ Microbiol: 2012, 78(24);8849-53
[PubMed:23064347]
[WorldCat.org]
[DOI]
(I p)
Ralf Moeller, Peter Setlow, Günther Reitz, Wayne L Nicholson
Roles of small, acid-soluble spore proteins and core water content in survival of Bacillus subtilis spores exposed to environmental solar UV radiation.
Appl Environ Microbiol: 2009, 75(16);5202-8
[PubMed:19542328]
[WorldCat.org]
[DOI]
(I p)
Barbara Setlow, Swaroopa Atluri, Ryan Kitchel, Kasia Koziol-Dube, Peter Setlow
Role of dipicolinic acid in resistance and stability of spores of Bacillus subtilis with or without DNA-protective alpha/beta-type small acid-soluble proteins.
J Bacteriol: 2006, 188(11);3740-7
[PubMed:16707666]
[WorldCat.org]
[DOI]
(P p)
Thierry Douki, Barbara Setlow, Peter Setlow
Effects of the binding of alpha/beta-type small, acid-soluble spore proteins on the photochemistry of DNA in spores of Bacillus subtilis and in vitro.
Photochem Photobiol: 2005, 81(1);163-9
[PubMed:15458366]
[WorldCat.org]
[DOI]
(P p)
B Setlow, K A McGinnis, K Ragkousi, P Setlow
Effects of major spore-specific DNA binding proteins on Bacillus subtilis sporulation and spore properties.
J Bacteriol: 2000, 182(24);6906-12
[PubMed:11092849]
[WorldCat.org]
[DOI]
(P p)
I Bagyan, J Hobot, S Cutting
A compartmentalized regulator of developmental gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(15);4500-7
[PubMed:8755877]
[WorldCat.org]
[DOI]
(P p)
B Setlow, P Setlow
Binding to DNA protects alpha/beta-type, small, acid-soluble spore proteins of Bacillus and Clostridium species against digestion by their specific protease as well as by other proteases.
J Bacteriol: 1995, 177(14);4149-51
[PubMed:7608092]
[WorldCat.org]
[DOI]
(P p)
P Fajardo-Cavazos, F Tovar-Rojo, P Setlow
Effect of promoter mutations and upstream deletions on the expression of genes coding for small, acid-soluble spore proteins of Bacillus subtilis.
J Bacteriol: 1991, 173(6);2011-6
[PubMed:1900507]
[WorldCat.org]
[DOI]
(P p)
J M Mason, P Fajardo-Cavazos, P Setlow
Levels of mRNAs which code for small, acid-soluble spore proteins and their LacZ gene fusions in sporulating cells of Bacillus subtilis.
Nucleic Acids Res: 1988, 16(14A);6567-83
[PubMed:2456528]
[WorldCat.org]
[DOI]
(P p)
R H Hackett, P Setlow
Properties of spores of Bacillus subtilis strains which lack the major small, acid-soluble protein.
J Bacteriol: 1988, 170(3);1403-4
[PubMed:3125155]
[WorldCat.org]
[DOI]
(P p)
J M Mason, P Setlow
Essential role of small, acid-soluble spore proteins in resistance of Bacillus subtilis spores to UV light.
J Bacteriol: 1986, 167(1);174-8
[PubMed:3087950]
[WorldCat.org]
[DOI]
(P p)
M J Connors, J M Mason, P Setlow
Cloning and nucleotide sequencing of genes for three small, acid-soluble proteins from Bacillus subtilis spores.
J Bacteriol: 1986, 166(2);417-25
[PubMed:3009398]
[WorldCat.org]
[DOI]
(P p)
