Difference between revisions of "Cah"
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|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to cephalosporin C | |style="background:#ABCDEF;" align="center"|'''Function''' || resistance to cephalosporin C | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03180 cah] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.419 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.419 | ||
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycgK]]'', ''[[ycgL]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycgK]]'', ''[[ycgL]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03180 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03180 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03180 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:cah_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:cah_context.gif]] | ||
Revision as of 12:06, 13 May 2013
- Description: cephalosporin C deacetylase
| Gene name | cah |
| Synonyms | |
| Essential | no |
| Product | cephalosporin C deacetylase) |
| Function | resistance to cephalosporin C |
| Gene expression levels in SubtiExpress: cah | |
| MW, pI | 35 kDa, 5.419 |
| Gene length, protein length | 954 bp, 318 aa |
| Immediate neighbours | ycgK, ycgL |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
resistance against toxins/ antibiotics
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU03180
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Deacetylation of xylans and xylo-oligosaccharides (according to Swiss-Prot)
- Protein family: carbohydrate esterase 7 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 1ODS
- UniProt: P94388
- KEGG entry: [3]
- E.C. number: 3.1.1.41
Additional information
Expression and regulation
- Operon: cah PubMed
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
A Takimoto, S Yagi, K Mitsushima
High-level expression, purification, and some properties of a recombinant cephalosporin-C deacetylase.
J Biosci Bioeng: 1999, 87(4);456-62
[PubMed:16232499]
[WorldCat.org]
[DOI]
(P p)
Akio Takimoto, Tomoaki Takakura, Hiroyoshi Tani, Shigeo Yagi, Kenji Mitsushima
Batch production of deacetyl 7-aminocephalosporanic acid by immobilized cephalosporin-C deacetylase.
Appl Microbiol Biotechnol: 2004, 65(3);263-7
[PubMed:15069587]
[WorldCat.org]
[DOI]
(P p)
Florence Vincent, Simon J Charnock, Koen H G Verschueren, Johan P Turkenburg, David J Scott, Wendy A Offen, Shirley Roberts, Gavin Pell, Harry J Gilbert, Gideon J Davies, James A Brannigan
Multifunctional xylooligosaccharide/cephalosporin C deacetylase revealed by the hexameric structure of the Bacillus subtilis enzyme at 1.9A resolution.
J Mol Biol: 2003, 330(3);593-606
[PubMed:12842474]
[WorldCat.org]
[DOI]
(P p)
K Mitsushima, A Takimoto, T Sonoyama, S Yagi
Gene cloning, nucleotide sequence, and expression of a cephalosporin-C deacetylase from Bacillus subtilis.
Appl Environ Microbiol: 1995, 61(6);2224-9
[PubMed:7793942]
[WorldCat.org]
[DOI]
(P p)
