Difference between revisions of "GerD"

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(Extended information on the protein)
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|style="background:#ABCDEF;" align="center"|'''Function''' || [[germination]]
 
|style="background:#ABCDEF;" align="center"|'''Function''' || [[germination]]
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU01550 gerD]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01550 gerD]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GerD GerD]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GerD GerD]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[salA]]'', ''[[kbaA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[salA]]'', ''[[kbaA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11931&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01550 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01550 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01550 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:gerD_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:gerD_context.gif]]

Revision as of 11:59, 13 May 2013

  • Description: required for clustering of germinant receptors in the spore inner membrane

Gene name gerD
Synonyms
Essential no
Product lipoprotein
Function germination
Gene expression levels in SubtiExpress: gerD
Interactions involving this protein in SubtInteract: GerD
MW, pI 20 kDa, 4.862
Gene length, protein length 555 bp, 185 aa
Immediate neighbours salA, kbaA
Sequences Protein DNA Advanced_DNA
Genetic context
GerD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GerD expression.png















Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon

The gene

Basic information

  • Locus tag: BSU01550

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: required for clustering of germinant receptors in the spore inner membrane PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • has an N-terminal signal sequence and a signal for fixation to lipid PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • outer surface of the inner spore membrane PubMed
    • inner spore membrane, spore coat, cortex, germ cell wall, some GerD is also present in the soluble fraction PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expression level depends on sporulation conditions (medim composition, temperature) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:

Labs working on this gene/protein

Anne Moir, University of Sheffield, UK

Your additional remarks

References

Additional references: PubMed

George Korza, Peter Setlow
Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane.
J Bacteriol: 2013, 195(7);1484-91
[PubMed:23335419] [WorldCat.org] [DOI] (I p)

Kerry-Ann V Stewart, Xuan Yi, Sonali Ghosh, Peter Setlow
Germination protein levels and rates of germination of spores of Bacillus subtilis with overexpressed or deleted genes encoding germination proteins.
J Bacteriol: 2012, 194(12);3156-64
[PubMed:22493018] [WorldCat.org] [DOI] (I p)

Arturo Ramirez-Peralta, Pengfei Zhang, Yong-Qing Li, Peter Setlow
Effects of sporulation conditions on the germination and germination protein levels of Bacillus subtilis spores.
Appl Environ Microbiol: 2012, 78(8);2689-97
[PubMed:22327596] [WorldCat.org] [DOI] (I p)

Xuan Yi, Jintao Liu, James R Faeder, Peter Setlow
Synergism between different germinant receptors in the germination of Bacillus subtilis spores.
J Bacteriol: 2011, 193(18);4664-71
[PubMed:21725007] [WorldCat.org] [DOI] (I p)

Keren K Griffiths, Jingqiao Zhang, Ann E Cowan, Ji Yu, Peter Setlow
Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster.
Mol Microbiol: 2011, 81(4);1061-77
[PubMed:21696470] [WorldCat.org] [DOI] (I p)

Wiyada Mongkolthanaruk, Carl Robinson, Anne Moir
Localization of the GerD spore germination protein in the Bacillus subtilis spore.
Microbiology (Reading): 2009, 155(Pt 4);1146-1151
[PubMed:19332816] [WorldCat.org] [DOI] (P p)

Patricia L Pelczar, Peter Setlow
Localization of the germination protein GerD to the inner membrane in Bacillus subtilis spores.
J Bacteriol: 2008, 190(16);5635-41
[PubMed:18556788] [WorldCat.org] [DOI] (I p)

Patricia L Pelczar, Takao Igarashi, Barbara Setlow, Peter Setlow
Role of GerD in germination of Bacillus subtilis spores.
J Bacteriol: 2007, 189(3);1090-8
[PubMed:17122337] [WorldCat.org] [DOI] (P p)

E H Kemp, R L Sammons, A Moir, D Sun, P Setlow
Analysis of transcriptional control of the gerD spore germination gene of Bacillus subtilis 168.
J Bacteriol: 1991, 173(15);4646-52
[PubMed:1906867] [WorldCat.org] [DOI] (P p)

J R Yon, R L Sammons, D A Smith
Cloning and sequencing of the gerD gene of Bacillus subtilis.
J Gen Microbiol: 1989, 135(12);3431-45
[PubMed:2517635] [WorldCat.org] [DOI] (P p)