Difference between revisions of "SspF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA
 
|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00450 sspF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00450 sspF]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 9.647   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 9.647   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[veg]]'', ''[[ispE]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[veg]]'', ''[[ispE]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11821&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00450 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:sspF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:sspF_context.gif]]

Revision as of 11:55, 13 May 2013

  • Description: small acid-soluble spore protein (minor)

Gene name sspF
Synonyms
Essential no
Product small acid-soluble spore protein (minor)
Function protection of spore DNA
Gene expression levels in SubtiExpress: sspF
MW, pI 6 kDa, 9.647
Gene length, protein length 183 bp, 61 aa
Immediate neighbours veg, ispE
Sequences Protein DNA Advanced_DNA
Genetic context
SspF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SspF expression.png
























Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

  • Locus tag: BSU00450

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: alpha/beta-type SASP family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: switched on during stage III of sporulation PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

C A Loshon, P Kraus, B Setlow, P Setlow
Effects of inactivation or overexpression of the sspF gene on properties of Bacillus subtilis spores.
J Bacteriol: 1997, 179(1);272-5
[PubMed:8982008] [WorldCat.org] [DOI] (P p)

M A Stephens, N Lang, K Sandman, R Losick
A promoter whose utilization is temporally regulated during sporulation in Bacillus subtilis.
J Mol Biol: 1984, 176(3);333-48
[PubMed:6205155] [WorldCat.org] [DOI] (P p)