Difference between revisions of "PfkA"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
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* essential {{PubMed|12682299}}, non-essential according to {{PubMed|23420519,20473954}}   
* essential according to  [http://www.ncbi.nlm.nih.gov/pubmed/12682299 Kobayashi'' et al.''], dispensable according to [http://www.ncbi.nlm.nih.gov/pubmed/20473954 Munoz-Marquez and Ponce-Rivas]
 
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pfkA_2986588_2987547_-1 pfkA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pfkA_2986588_2987547_-1 pfkA] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Mutant:'''
 
* '''Mutant:'''
* GP590 (''pfkA''::''cat''), available in [[Stülke]] lab
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* GP590 (''pfkA''::''cat''), available in [[Jörg Stülke]]'s lab
* GP595 (''pfkA''::''erm''), available in [[Stülke]] lab
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* GP595 (''pfkA''::''erm''), available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''  
 
* '''Expression vector:'''  
** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP87, available in [[Stülke]] lab
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** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP87, available in [[Jörg Stülke]]'s lab
** for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], in [[pGP382]]: pGP1266, available in [[Stülke]] lab
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** for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], in [[pGP382]]: pGP1266, available in [[Jörg Stülke]]'s lab
** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP393, available in [[Stülke]] lab
+
** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP393, available in [[Jörg Stülke]]'s lab
** for expression in ''B. subtilis'', in [[pBQ200]]: pGP1422, available in [[Stülke]] lab
+
** for expression in ''B. subtilis'', in [[pBQ200]]: pGP1422, available in [[Jörg Stülke]]'s lab
 
            
 
            
* '''lacZ fusion:''' pGP511 (in [[pAC6]]), available in [[Stülke]] lab
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* '''lacZ fusion:''' pGP511 (in [[pAC6]]), available in [[Jörg Stülke]]'s lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
  
* '''FLAG-tag construct:''' GP1019 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
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* '''FLAG-tag construct:''' GP1019 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''
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=References=
 
=References=
 
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<pubmed> 16479537,19193632, 11489127, 8136379, 7873536 4269800 20473954 20572937 ,21803996 22198292 22198292 22517742 23420519</pubmed>
<pubmed> 16479537,19193632, 11489127, 8136379, 7873536 4269800 20473954 20572937 ,21803996 22198292 22198292 22517742</pubmed>
 
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:40, 14 April 2013

  • Description: phosphofructokinase, glycolytic enzyme

Gene name pfkA
Synonyms pfk
Essential no
Product 6-phosphofructokinase
Function catabolic enzyme in glycolysis
Gene expression levels in SubtiExpress: pfkA
Interactions involving this protein in SubtInteract: PfkA
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism, Sugar catabolism
MW, pI 34,1 kDa, 6.14
Gene length, protein length 957 bp, 319 amino acids
Immediate neighbours pyk, accA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PfkA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PfkA expression.png















Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29190

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)
  • Protein family: phosphofructokinase family (according to Swiss-Prot) phosphofructokinase family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Allosteric Regulation (Reversible) PubMed
  • Domains:
    • 3 x nucleotide binding domain (ATP) (21–25), (154–158), (171–187)
  • Modification:
    • phosphorylated on Arg-233 PubMed
  • Cofactor(s): Mg2+
  • Effectors of protein activity:
    • Inhibited by citrate, PEP (Hill Coefficient 3) and Ca2+ (competes with Mg2+) in B. licheniformes PubMed.
    • Inhibited by ATP (competitively) and f6p (non-competitively) in G. stearothermophillus PubMed
    • Activated by GDP and ADP in lower concentrations (1mM); above that inhibition, competing with the ATP for the binding site (in G. stearothermophillus) PubMed
    • Activated by NH4+ PubMed

Database entries

  • Structure:
    • 4A3S PubMed
    • 1MTO (mutant, complex with fructose-6-phosphate, Geobacillus stearothermophilus)
    • 4PFK (Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • twofold induced by glucose PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP87, available in Jörg Stülke's lab
    • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1266, available in Jörg Stülke's lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP393, available in Jörg Stülke's lab
    • for expression in B. subtilis, in pBQ200: pGP1422, available in Jörg Stülke's lab
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References