Difference between revisions of "PfkA"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
− | + | * essential {{PubMed|12682299}}, non-essential according to {{PubMed|23420519,20473954}} | |
− | * essential | ||
=== Database entries === | === Database entries === | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pfkA_2986588_2987547_-1 pfkA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pfkA_2986588_2987547_-1 pfkA] {{PubMed|22383849}} | ||
− | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Mutant:''' | * '''Mutant:''' | ||
− | * GP590 (''pfkA''::''cat''), available in [[Stülke]] lab | + | * GP590 (''pfkA''::''cat''), available in [[Jörg Stülke]]'s lab |
− | * GP595 (''pfkA''::''erm''), available in [[Stülke]] lab | + | * GP595 (''pfkA''::''erm''), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
− | ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP87, available in [[Stülke]] lab | + | ** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP87, available in [[Jörg Stülke]]'s lab |
− | ** for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], in [[pGP382]]: pGP1266, available in [[Stülke]] lab | + | ** for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], in [[pGP382]]: pGP1266, available in [[Jörg Stülke]]'s lab |
− | ** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP393, available in [[Stülke]] lab | + | ** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP393, available in [[Jörg Stülke]]'s lab |
− | ** for expression in ''B. subtilis'', in [[pBQ200]]: pGP1422, available in [[Stülke]] lab | + | ** for expression in ''B. subtilis'', in [[pBQ200]]: pGP1422, available in [[Jörg Stülke]]'s lab |
− | * '''lacZ fusion:''' pGP511 (in [[pAC6]]), available in [[Stülke]] lab | + | * '''lacZ fusion:''' pGP511 (in [[pAC6]]), available in [[Jörg Stülke]]'s lab |
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
− | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab | + | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab |
− | * '''FLAG-tag construct:''' GP1019 (spc, based on [[pGP1331]]), available in | + | * '''FLAG-tag construct:''' GP1019 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
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=References= | =References= | ||
− | + | <pubmed> 16479537,19193632, 11489127, 8136379, 7873536 4269800 20473954 20572937 ,21803996 22198292 22198292 22517742 23420519</pubmed> | |
− | <pubmed> 16479537,19193632, 11489127, 8136379, 7873536 4269800 20473954 20572937 ,21803996 22198292 22198292 22517742</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 16:40, 14 April 2013
- Description: phosphofructokinase, glycolytic enzyme
Gene name | pfkA |
Synonyms | pfk |
Essential | no |
Product | 6-phosphofructokinase |
Function | catabolic enzyme in glycolysis |
Gene expression levels in SubtiExpress: pfkA | |
Interactions involving this protein in SubtInteract: PfkA | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism | |
MW, pI | 34,1 kDa, 6.14 |
Gene length, protein length | 957 bp, 319 amino acids |
Immediate neighbours | pyk, accA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29190
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)
- Protein family: phosphofructokinase family (according to Swiss-Prot) phosphofructokinase family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Allosteric Regulation (Reversible) PubMed
- Domains:
- 3 x nucleotide binding domain (ATP) (21–25), (154–158), (171–187)
- Modification:
- phosphorylated on Arg-233 PubMed
- Cofactor(s): Mg2+
- Effectors of protein activity:
- Inhibited by citrate, PEP (Hill Coefficient 3) and Ca2+ (competes with Mg2+) in B. licheniformes PubMed.
- Inhibited by ATP (competitively) and f6p (non-competitively) in G. stearothermophillus PubMed
- Activated by GDP and ADP in lower concentrations (1mM); above that inhibition, competing with the ATP for the binding site (in G. stearothermophillus) PubMed
- Activated by NH4+ PubMed
- Localization: cytoplasm (Homogeneous) PubMed
Database entries
- Structure:
- UniProt: O34529
- KEGG entry: [3]
- E.C. number: 2.7.1.11
Additional information
- PfkA is a moonlighting protein. PubMed
- extensive information on the structure and enzymatic properties of PfkA can be found at Proteopedia
Expression and regulation
- Regulation:
- twofold induced by glucose PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP590 (pfkA::cat), available in Jörg Stülke's lab
- GP595 (pfkA::erm), available in Jörg Stülke's lab
- Expression vector:
- for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP87, available in Jörg Stülke's lab
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1266, available in Jörg Stülke's lab
- for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP393, available in Jörg Stülke's lab
- for expression in B. subtilis, in pBQ200: pGP1422, available in Jörg Stülke's lab
- lacZ fusion: pGP511 (in pAC6), available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct: GP1019 (spc, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References