Difference between revisions of "FrlO"

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* '''Operon:'''  
 
* '''Operon:'''  
 
** ''[[yurR]]-[[yurQ]]-[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' {{PubMed|12823818}}
 
** ''[[yurR]]-[[yurQ]]-[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' {{PubMed|12823818}}
** ''[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' {{PubMed|21347729}}
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** ''[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' {{PubMed|23175651,21347729}}
 +
** ''[[frlB]]-[[frlO]]'' {{PubMed|23175651}}
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=frlO_3349761_3351029_-1 frlO] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=frlO_3349761_3351029_-1 frlO] {{PubMed|22383849}}
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* '''Additional information:'''
 
* '''Additional information:'''
 
** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
 
** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
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** the ''[[frlB]]-[[frlO]]-[[frlN]]-[[frlM]]-[[frlD]]'' operon is not expressed in a ''[[cshA]]'' mutant {{PubMed|23175651}}
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
 
'''Additional references:''' {{PubMed|21397556}}
 
'''Additional references:''' {{PubMed|21397556}}
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<pubmed> 23175651 </pubmed>
 
  <big>''Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J''  </big>
 
  <big>''Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J''  </big>
 
  <big>'''RNA processing in ''Bacillus subtilis'': identification of targets of the essential RNase Y.''' </big>
 
  <big>'''RNA processing in ''Bacillus subtilis'': identification of targets of the essential RNase Y.''' </big>

Revision as of 17:11, 26 November 2012

Gene name frlO
Synonyms yurO
Essential no
Product aminosugar ABC transporter (binding protein)
Function uptake of sugar amines
Gene expression levels in SubtiExpress: frlO
Interactions involving this protein in SubtInteract: FrlO
Metabolic function and regulation of this protein in SubtiPathways:
Alternative nitrogen sources
MW, pI 47 kDa, 5.674
Gene length, protein length 1266 bp, 422 aa
Immediate neighbours frlN, frlB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YurO context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FrlO expression.png















Categories containing this gene/protein

ABC transporters, utilization of specific carbon sources, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon, FrlR regulon

The gene

Basic information

  • Locus tag: BSU32600

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: bacterial solute-binding protein 1 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • frlB: repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • frlB: induced in the presence of fructosamine (FrlR) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional references: PubMed

Martin Lehnik-Habrink, Leonie Rempeters, Ákos T Kovács, Christoph Wrede, Claudia Baierlein, Heike Krebber, Oscar P Kuipers, Jörg Stülke
DEAD-Box RNA helicases in Bacillus subtilis have multiple functions and act independently from each other.
J Bacteriol: 2013, 195(3);534-44
[PubMed:23175651] [WorldCat.org] [DOI] (I p)

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947