Difference between revisions of "CshB"
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| − | * '''Description:''' [[DEAD-box RNA helicases|DEAD-box RNA helicase]]<br/><br/> | + | * '''Description:''' [[DEAD-box RNA helicases|DEAD-box RNA helicase]], important for adaptation to low temperatures<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 68: | Line 68: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' RNA helicase | + | * '''Catalyzed reaction/ biological activity:''' |
| + | ** RNA helicase | ||
| + | ** required for [[ribosome]] assembly {{PubMed|23175651}} | ||
* '''Protein family:''' [[DEAD-box RNA helicases|DEAD-box RNA helicase]] | * '''Protein family:''' [[DEAD-box RNA helicases|DEAD-box RNA helicase]] | ||
| Line 114: | Line 116: | ||
* '''Regulation:''' | * '''Regulation:''' | ||
** well expressed in complex medium, poorly expressed in minimal medium {{PubMed|20572937}} | ** well expressed in complex medium, poorly expressed in minimal medium {{PubMed|20572937}} | ||
| − | ** expression declines in stationary phase {{PubMed|20572937}} | + | ** expression declines in stationary phase {{PubMed|23175651,20572937}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
Revision as of 16:47, 26 November 2012
- Description: DEAD-box RNA helicase, important for adaptation to low temperatures
| Gene name | cshB |
| Synonyms | yqfR |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| Gene expression levels in SubtiExpress: cshB | |
| Interactions involving this protein in SubtInteract: CshB | |
| MW, pI | 49 kDa, 9.89 |
| Gene length, protein length | 1314 bp, 438 aa |
| Immediate neighbours | nfo, yqfQ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DEAD-box RNA helicases, translation
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU25140
Phenotypes of a mutant
- poor growth at low temperatures (16 to 20°C) PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Protein family: DEAD-box RNA helicase
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasma, colocalizes with the ribosomes PubMed
Database entries
- Structure:
- UniProt: P54475
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP1051 (cat), available in Jörg Stülke's lab
- Expression vector:
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1064 (spc), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1011 (spc, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
Your additional remarks
References
Additional publications: PubMed
