RocC

• Description: amino acid permease
  
  

Gene name	rocC
Synonyms	ipa-78d
Essential	no
Product	amino acid permease
Function	arginine, ornithine and citrulline uptake
Gene expression levels in SubtiExpress: rocC
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	51 kDa, 8.41
Gene length, protein length	1410 bp, 470 aa
Immediate neighbours	ywfA, rocB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids, membrane proteins

This gene is a member of the following regulons

AhrC regulon, CodY regulon, RocR regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU37760

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: secondary amino acid tranporter

• Paralogous protein(s): AapA, GabP, HutM, RocC, RocE, YbgF, YbxG, YdgF, YtnA, YvbW

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39636

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rocA-rocB-rocC PubMed

• Expression browser: rocC PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • induction by arginine (RocR, AhrC) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • RocR: transcription activation PubMed
  • AhrC: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

U Klingel, C M Miller, A K North, P G Stockley, S Baumberg
A binding site for activation by the Bacillus subtilis AhrC protein, a repressor/activator of arginine metabolism.
Mol Gen Genet: 1995, 248(3);329-40
[PubMed:7565595] [WorldCat.org] [DOI] (P p)

S Calogero, R Gardan, P Glaser, J Schweizer, G Rapoport, M Debarbouille
RocR, a novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators.
J Bacteriol: 1994, 176(5);1234-41
[PubMed:8113162] [WorldCat.org] [DOI] (P p)