Difference between revisions of "YtsJ"
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=Biological materials = | =Biological materials = | ||
− | * '''Mutant:''' GP612 (spc), available in [[Stülke]] lab | + | * '''Mutant:''' |
+ | **GP612 (spc), available in [[Stülke]] lab | ||
+ | **GP1143 (spc), available in [[Stülke]] lab | ||
* '''Expression vector:''' | * '''Expression vector:''' |
Revision as of 08:05, 30 October 2012
- Description: malic enzyme, forms a transhydrogenation cycle with MalS for balancing of NADPH
Gene name | ytsJ |
Synonyms | |
Essential | no |
Product | NADP-dependent malate dehydrogenase |
Function | malate utilization |
Gene expression levels in SubtiExpress: ytsJ | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 43 kDa, 5.046 |
Gene length, protein length | 1230 bp, 410 aa |
Immediate neighbours | accD, dnaE |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
utilization of specific carbon sources
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29220
Phenotypes of a mutant
Poor growth with malate as single carbon source PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate + NADP+ = pyruvate + CO2 + NADPH (according to Swiss-Prot) malate <--> pyruvate
- Protein family: malic enzymes family (according to Swiss-Prot)
- Paralogous protein(s): MleA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
Database entries
- Structure: 1WW8 (from Pyrococcus horikoshii, 46% identity, 63% similarity)
- UniProt: O34962
- KEGG entry: [3]
- E.C. number: 1.1.1.38
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Your additional remarks
References