Difference between revisions of "YtsJ"

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(Biological materials)
(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' GP612 (spc), available in [[Stülke]] lab
+
* '''Mutant:'''  
 +
**GP612 (spc), available in [[Stülke]] lab
 +
**GP1143 (spc), available in [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 08:05, 30 October 2012

  • Description: malic enzyme, forms a transhydrogenation cycle with MalS for balancing of NADPH

Gene name ytsJ
Synonyms
Essential no
Product NADP-dependent malate dehydrogenase
Function malate utilization
Gene expression levels in SubtiExpress: ytsJ
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 43 kDa, 5.046
Gene length, protein length 1230 bp, 410 aa
Immediate neighbours accD, dnaE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YtsJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YtsJ expression.png




























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29220

Phenotypes of a mutant

Poor growth with malate as single carbon source PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate + NADP+ = pyruvate + CO2 + NADPH (according to Swiss-Prot) malate <--> pyruvate
  • Protein family: malic enzymes family (according to Swiss-Prot)
  • Paralogous protein(s): MleA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: Cytoplasm (Homogeneous) PubMed cytoplasm protein PubMed

Database entries

  • Structure: 1WW8 (from Pyrococcus horikoshii, 46% identity, 63% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • GP612 (spc), available in Stülke lab
    • GP1143 (spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • FLAG-tag construct: GP1131 (spc, based on pGP1331), available in the Stülke lab
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References