YcnK

• Description: copper-responsive transcription repressor of the ycnK-ycnJ-ycnI operon
  
  

Gene name	ycnK
Synonyms
Essential	no
Product	transcription repressor, DeoR family
Function	regulation of copper uptake
Gene expression levels in SubtiExpress: ycnK
MW, pI	21 kDa, 7.371
Gene length, protein length	570 bp, 190 aa
Immediate neighbours	ycnJ, ycnL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), transcription factors and their control

This gene is a member of the following regulons

AbrB regulon, YcnK regulon

The YcnK regulon:

• ycnK-ycnJ-ycnI

The gene

Basic information

• Locus tag: BSU03960

Phenotypes of a mutant

• elevated expression of ycnJ under conditions of copper excess PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: repression of ycnK-ycnJ-ycnI expression in the presence of excess copper PubMed

• Protein family: DeoR family of transcription factors

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): copper acts as corepressor PubMed

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed

• Localization:

Database entries

• Structure:

• UniProt: P94433

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ycnK-ycnJ-ycnI PubMed

• Expression browser: ycnK PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during logrithmic growth (AbrB) PubMed
  • induced by copper limitation (YcnK) PubMed
  • induction of the ycnK-ycnJ-ycnI in a csoR mutant results from intracellular copper depletion due to the constitutive expression of the copZ-copA operon (copper export) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • YcnK: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

References

Reviews

Corinna Rademacher, Bernd Masepohl
Copper-responsive gene regulation in bacteria.
Microbiology (Reading): 2012, 158(Pt 10);2451-2464
[PubMed:22918892] [WorldCat.org] [DOI] (I p)