MleA

• Description: malic enzyme
  
  

Gene name	mleA
Synonyms	yqkJ
Essential	no
Product	NAD-dependent malate dehydrogenase
Function	malate utilization
Gene expression levels in SubtiExpress: mleA
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	45 kDa, 4.895
Gene length, protein length	1317 bp, 439 aa
Immediate neighbours	yqkK, mleN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AnsR regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU23550

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate + NAD⁺ = pyruvate + CO₂ + NADH (according to Swiss-Prot) malate --> pyruvate

• Protein family: malic enzymes family (according to Swiss-Prot)

• Paralogous protein(s): YtsJ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54572

• KEGG entry: [2]

• E.C. number: 1.1.1.38

Expression and regulation

• Operon:
  • ansA-ansB-mleN-mleA PubMed
  • mleN-mleA PubMed

• Expression browser: mleA PubMed

• Sigma factor:
  • ansA: SigA PubMed
  • mleN: SigA PubMed

• Regulation:
  • ansA: expressed in the presence of asparagine (AnsR) PubMed
  • mleN: repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • AnsR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1136 (cat) available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: