Difference between revisions of "MleA"
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
− | + | {{SubtiWiki regulon|[[AnsR regulon]]}}, | |
+ | {{SubtiWiki regulon|[[CcpA regulon]]}} | ||
=The gene= | =The gene= | ||
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=== Additional information=== | === Additional information=== | ||
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* '''Operon:''' | * '''Operon:''' | ||
+ | ** ''[[ansA]]-[[ansB]]-[[mleN]]-[[mleA]]'' {{PubMed|22383849}} | ||
+ | ** ''[[mleN]]-[[mleA]]'' {{PubMed|22383849}} | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mleA_2451463_2452782_-1 mleA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mleA_2451463_2452782_-1 mleA] {{PubMed|22383849}} | ||
* '''Sigma factor:''' | * '''Sigma factor:''' | ||
+ | ** ''[[mleN]]'': [[SigA]] {{PubMed|22900538}} | ||
* '''Regulation:''' | * '''Regulation:''' | ||
+ | ** repressed by glucose ([[CcpA]]) {{PubMed|22900538}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
+ | ** [[CcpA]]: transcription repression {{PubMed|22900538}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
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=References= | =References= | ||
− | <pubmed> 16788182 </pubmed> | + | <pubmed> 16788182 22383849 22900538</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 16:07, 23 August 2012
- Description: malic enzyme
Gene name | mleA |
Synonyms | yqkJ |
Essential | no |
Product | NAD-dependent malate dehydrogenase |
Function | malate utilization |
Gene expression levels in SubtiExpress: mleA | |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 45 kDa, 4.895 |
Gene length, protein length | 1317 bp, 439 aa |
Immediate neighbours | yqkK, mleN |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
utilization of specific carbon sources
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU23550
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate --> pyruvate
- Protein family: malic enzymes family (according to Swiss-Prot)
- Paralogous protein(s): YtsJ
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P54572
- KEGG entry: [2]
- E.C. number: 1.1.1.38
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant: GP1136 (cat) available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538]
[WorldCat.org]
[DOI]
(I e)
Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849]
[WorldCat.org]
[DOI]
(I p)
Guillaume Lerondel, Thierry Doan, Nicola Zamboni, Uwe Sauer, Stéphane Aymerich
YtsJ has the major physiological role of the four paralogous malic enzyme isoforms in Bacillus subtilis.
J Bacteriol: 2006, 188(13);4727-36
[PubMed:16788182]
[WorldCat.org]
[DOI]
(P p)