GabD

• Description: succinate-semialdehyde dehydrogenase (NADP), general stress protein
  
  

Gene name	gabD
Synonyms	ycnH
Essential	no
Product	succinate-semialdehyde dehydrogenase (NADP)
Function	utilization of gamma-amino butyric acid
Gene expression levels in SubtiExpress: gabD
MW, pI	50 kDa, 4.763
Gene length, protein length	1386 bp, 462 aa
Immediate neighbours	gabT, glcU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

GabR regulon, SigB regulon

The gene

Basic information

• Locus tag: BSU03910

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): YfmT, AldY, YcbD, GbsA, DhaS, IolA, PutC, RocA, AldX, YwdH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3RHH (from from Bacillus halodurans C-125 complexed with NADP, 327identity, 68% similarity)

• UniProt: P94428

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • gabT-gabD PubMed
  • gabD PubMed

• Expression browser: gabD PubMed

• Sigma factor:
  • gabT: SigA PubMed
  • gabD: SigB PubMed

• Regulation:
  • gabT: expressed in the presence of gamma-butyric acid (GabR) PubMed
  • gabD: induced by stress (SigB) PubMed

• Regulatory mechanism:
  • GabR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Boris R Belitsky
Bacillus subtilis GabR, a protein with DNA-binding and aminotransferase domains, is a PLP-dependent transcriptional regulator.
J Mol Biol: 2004, 340(4);655-64
[PubMed:15223311] [WorldCat.org] [DOI] (P p)

Boris R Belitsky, Abraham L Sonenshein
GabR, a member of a novel protein family, regulates the utilization of gamma-aminobutyrate in Bacillus subtilis.
Mol Microbiol: 2002, 45(2);569-83
[PubMed:12123465] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)