Difference between revisions of "CshA"
m (Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk) |
|||
| Line 1: | Line 1: | ||
| − | + | * '''Description:''' [[DEAD-box RNA helicases|DEAD-box RNA helicase]], important for adaptation to low temperatures<br/><br/> | |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 13: | Line 13: | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase | |style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase | ||
| − | |||
| − | |||
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CshA CshA] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CshA CshA] | ||
| Line 28: | Line 26: | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ydbR_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ydbR_context.gif]] | ||
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
| − | |||
| − | |||
|- | |- | ||
|} | |} | ||
__TOC__ | __TOC__ | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
<br/><br/> | <br/><br/> | ||
| Line 119: | Line 109: | ||
* '''Operon:''' | * '''Operon:''' | ||
| − | |||
| − | |||
* '''Sigma factor:''' | * '''Sigma factor:''' | ||
| Line 130: | Line 118: | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 11:21, 7 August 2012
- Description: DEAD-box RNA helicase, important for adaptation to low temperatures
| Gene name | cshA |
| Synonyms | ydbR |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| Interactions involving this protein in SubtInteract: CshA | |
| MW, pI | 57 kDa, 9.89 |
| Gene length, protein length | 1533 bp, 511 aa |
| Immediate neighbours | murF, ydbS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
DEAD-box RNA helicases, cold stress proteins, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04580
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: RNA helicase
- Protein family: helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicase
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasma, colocalizes with the ribosomes PubMed, cell membrane PubMed
Database entries
- Structure:
- UniProt: P96614
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1387, available in Stülke lab
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1026, available in Stülke lab
- for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP1386, available in Stülke lab
- lacZ fusion:
- GFP fusion: pGP1369 for chromosomal expression of CshA-YFP, available in Stülke lab
- B. subtilis GP1081 cshA-gfp spc, available in Stülke lab,
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
Your additional remarks
References
Additional publications: PubMed
