TrmB

• Description: tRNA (m7G46) methyltransferase
  
  

Gene name	trmB
Synonyms	ytmQ
Essential	no
Product	tRNA (m7G46) methyltransferase
Function	tRNA modification
MW, pI	24 kDa, 6.351
Gene length, protein length	639 bp, 213 aa
Immediate neighbours	ytnP, ytzH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU29900

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine (according to Swiss-Prot)

• Protein family: TrmB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2FCA

• UniProt: O34522

• KEGG entry: [3]

• E.C. number: 2.1.1.33

Expression and regulation

• Operon: trmB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ingrid Zegers, Daniel Gigot, Françoise van Vliet, Catherine Tricot, Stéphane Aymerich, Janusz M Bujnicki, Jan Kosinski, Louis Droogmans
Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase.
Nucleic Acids Res: 2006, 34(6);1925-34
[PubMed:16600901] [WorldCat.org] [DOI] (I e)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)