trmB

trmB
168

tRNA (m7G46) methyltransferase

Locus
BSU_29900
Molecular weight
24.36 kDa
Isoelectric point
6.35
Protein length
213 aaSequenceBlast
Gene length
642 bpSequenceBlast
Function
tRNA modification
Product
tRNA (m7G46) methyltransferase
Essential
no
E.C.
2.1.1.33
Synonyms
trmB, ytmQ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0220 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,059,547  3,060,188
The protein
Catalyzed reaction/ biological activity
guanosine46 in tRNA + S-adenosyl-L-methionine --> N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
Methyltransferase superfamily (according to UniProt)
class I-like SAM-binding methyltransferase superfamily (according to UniProt)
Cofactors
S-adenosylmethionine PubMed
Structure
2FCA (PDB) PubMed
O34522 (AlphaFold)
Expression and Regulation
Operons
Genes
trmB
Description
PubMed
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trmB

2025-04-03 11:27:06

ghost

231

436d8185ca5910684a763ce7ebe2c9dce2a7051c

CB2035A420E61DFF5966A2C89C85978844D6109D

Biological materials
Mutant
GP2649 (trmB::cat), available in Jörg Stülke's lab
MGNA-A435 (ytmQ::erm), available at the NBRP B. subtilis, Japan
BKE29900 (trmB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGATTGACACCTCTAT,  downstream forward: _UP4_TAAAGACAGACTCTGACAGG
BKK29900 (trmB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGATTGACACCTCTAT,  downstream forward: _UP4_TAAAGACAGACTCTGACAGG
References
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Page visits: 3366

Time of last update: 2025-04-02 03:07:21

Author of last update: Jstuelk