Difference between revisions of "HypO"
| Line 4: | Line 4: | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |''yfkO'' | + | |''yfkO'' (hypO) |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''hypO'' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
| Line 12: | Line 12: | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || NAD(P)H-flavin nitroreductase | |style="background:#ABCDEF;" align="center"| '''Product''' || NAD(P)H-flavin nitroreductase | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || probably involved in NaOCl and diamide detoxification |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 5.667 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 5.667 | ||
| Line 36: | Line 36: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| + | '''The ''[[yybR]]'' (hypR) regulon | ||
=The gene= | =The gene= | ||
| Line 96: | Line 97: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''ykfO'' (according to [http://dbtbs.hgc.jp/COG/prom/yfkO.html DBTBS]) | + | * '''Operon:''' ''ykfO'' (hypO) (according to [http://dbtbs.hgc.jp/COG/prom/yfkO.html DBTBS]) |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| − | * '''Regulation:''' | + | * '''Regulation:''' induced under disulfide stress conditions (diamide, NaOCl) |
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' induction of YfkO (HypO) positively controlled by the MarR/DUF24-family regulator YybR (HypR) |
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 126: | Line 127: | ||
=References= | =References= | ||
<pubmed> 20727918 </pubmed> | <pubmed> 20727918 </pubmed> | ||
| + | |||
| + | Palm, Gottfried; Chi, Bui; Waack, Paul; Gronau, Katrin; Becher, Dörte; Albrecht, Dirk; Hinrichs, Winfried; Read, Randy; Antelmann, Haike | ||
| + | Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR | ||
| + | Nucleic Acid Research 2012, in press | ||
| + | |||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:55, 26 December 2011
- Description: NAD(P)H-flavin nitroreductase
| Gene name | yfkO (hypO) |
| Synonyms | hypO |
| Essential | no |
| Product | NAD(P)H-flavin nitroreductase |
| Function | probably involved in NaOCl and diamide detoxification |
| MW, pI | 25 kDa, 5.667 |
| Gene length, protein length | 663 bp, 221 aa |
| Immediate neighbours | treR, yfkN |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The yybR (hypR) regulon
The gene
Basic information
- Locus tag: BSU07830
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: nitroreductase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O34475
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: ykfO (hypO) (according to DBTBS)
- Regulation: induced under disulfide stress conditions (diamide, NaOCl)
- Regulatory mechanism: induction of YfkO (HypO) positively controlled by the MarR/DUF24-family regulator YybR (HypR)
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
G A Prosser, A V Patterson, D F Ackerley
uvrB gene deletion enhances SOS chromotest sensitivity for nitroreductases that preferentially generate the 4-hydroxylamine metabolite of the anti-cancer prodrug CB1954.
J Biotechnol: 2010, 150(1);190-4
[PubMed:20727918]
[WorldCat.org]
[DOI]
(I p)
Palm, Gottfried; Chi, Bui; Waack, Paul; Gronau, Katrin; Becher, Dörte; Albrecht, Dirk; Hinrichs, Winfried; Read, Randy; Antelmann, Haike
Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR
Nucleic Acid Research 2012, in press
