GmuF

• Description: mannose-6-P-isomerase
  
  

Gene name	gmuF
Synonyms	ydhS
Essential	no
Product	mannose-6-P-isomerase
Function	glucomannan utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	35 kDa, 5.895
Gene length, protein length	945 bp, 315 aa
Immediate neighbours	gmuE, gmuG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, GmuR regulon

The gene

Basic information

• Locus tag: BSU05870

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O05511

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by conjac glucomannan (requires GmuG) (GmuR) PubMed
  • induced by cellobiose (GmuR) PubMed
  • subject to carbon catabolite repression (CcpA) PubMed

• Regulatory mechanism:
  • GmuR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Soo-Jin Yeom, Jung-Hwan Ji, Nam-Hee Kim, Chang-Su Park, Deok-Kun Oh
Substrate specificity of a mannose-6-phosphate isomerase from Bacillus subtilis and its application in the production of L-ribose.
Appl Environ Microbiol: 2009, 75(14);4705-10
[PubMed:19447949] [WorldCat.org] [DOI] (I p)

Yoshito Sadaie, Hisashi Nakadate, Reiko Fukui, Lii Mien Yee, Kei Asai
Glucomannan utilization operon of Bacillus subtilis.
FEMS Microbiol Lett: 2008, 279(1);103-9
[PubMed:18177310] [WorldCat.org] [DOI] (P p)