Difference between revisions of "McsA"
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' ''mcsA::aphA3'' available from the Gerth lab |
| − | * '''Expression vector:''' | + | * '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
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* '''two-hybrid system:''' | * '''two-hybrid system:''' | ||
| − | * '''Antibody:''' | + | * '''Antibody:''' available in Gerth lab |
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
Revision as of 19:52, 6 September 2011
| Gene name | mcsA |
| Synonyms | yacH |
| Essential | no |
| Product | activator of McsB kinase activity |
| Function | control of CtsR activity |
| Interactions involving this protein in SubtInteract: McsA | |
| Metabolic function and regulation of this protein in SubtiPathways: Stress | |
| MW, pI | 20 kDa, 6.624 |
| Gene length, protein length | 555 bp, 185 aa |
| Immediate neighbours | ctsR, mcsB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
transcription factors and their control, sporulation proteins, general stress proteins (controlled by SigB), heat shock proteins
This gene is a member of the following regulons
CtsR regulon, SigB regulon, SigF regulon
The gene
Basic information
- Locus tag: BSU00840
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P37569
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant: mcsA::aphA3 available from the Gerth lab
- Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody: available in Gerth lab
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
