YcbD

• Description: NADP(+)-dependent alpha-ketoglutaric semialdehyde dehydrogenase
  
  

Gene name	ycbD
Synonyms
Essential	no
Product	NADP(+)-dependent alpha-ketoglutaric semialdehyde dehydrogenase
Function	utilization of D-glucarate/galactarate
MW, pI	52 kDa, 4.787
Gene length, protein length	1464 bp, 488 aa
Immediate neighbours	ycbC, ycbE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

YcbG regulon

The gene

Basic information

• Locus tag: BSU02470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: an aldehyde + NAD⁺ + H₂O = an acid + NADH (according to Swiss-Prot)

• Protein family: aldehyde dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): YfmT, AldY, GabD, GbsA, DhaS, IolA, YcgN, RocA, AldX, YwdH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3RHH (from from Bacillus halodurans C-125 complexed with NADP, 36% identity, 69% similarity)

• UniProt: P42236

• KEGG entry: [3]

• E.C. number: 1.2.1.3

Expression and regulation

• Operon ycbC-ycbD-ycbE-ycbF-ycbG-ycbH-ycbJ PubMed

• Sigma factor:

• Regulation: induced by D-glucarate/galactarate (YcbG) PubMed

• Regulatory mechanism: YcbG: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Seiya Watanabe, Masaki Yamada, Iwao Ohtsu, Keisuke Makino
alpha-ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate, D-galactarate, and hydroxy-L-proline. Molecular and metabolic convergent evolution.
J Biol Chem: 2007, 282(9);6685-95
[PubMed:17202142] [WorldCat.org] [DOI] (P p)

Shigeo Hosoya, Kunio Yamane, Michio Takeuchi, Tsutomu Sato
Identification and characterization of the Bacillus subtilis D-glucarate/galactarate utilization operon ycbCDEFGHJ.
FEMS Microbiol Lett: 2002, 210(2);193-9
[PubMed:12044674] [WorldCat.org] [DOI] (P p)