FruA

• Description: fructose-specific phosphotransferase system, EIIABC of the PTS
  
  

Gene name	fruA
Synonyms
Essential	no
Product	fructose-specific phosphotransferase system, EIIABC
Function	fructose uptake and phosphorylation
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	67 kDa, 5.241
Gene length, protein length	1905 bp, 635 aa
Immediate neighbours	fruK, sipT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, membrane proteins, phosphoproteins

This gene is a member of the following regulons

FruR regulon

The gene

Basic information

• Locus tag: BSU14400

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

• Protein family: PTS permease, fructose/ mannitol permease (Fru) family PubMed

• Paralogous protein(s): ManP

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • FruA-HPr
  • FruA is a member of a suspected group of hubs proteins that were suggested to be involved in a large number of interactions PubMed

• Localization: cell membrane (homogeneous) PubMed

Database entries

• Structure: 2R4Q

• UniProt: P71012

• KEGG entry: [3]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: fruR-fruK-fruA (according to DBTBS)

• Sigma factor:

• Regulation:
  • induced in the presence of fructose (FruR) (according to DBTBS)

• Regulatory mechanism:
  • FruR: transcription repression (according to DBTBS)

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: