KinB

• Description: two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay
  
  

Gene name	kinB
Synonyms
Essential	no
Product	two-component sensor kinase
Function	initiation of sporulation
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	47 kDa, 6.682
Gene length, protein length	1287 bp, 429 aa
Immediate neighbours	patB, kapB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU31450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, membrane proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • autophosphorylation, phosphorylation of Spo0F
  • mainly active in the older, inner regions of a colony (with KinA) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: six transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions: KinB-Sda, KinB-Spo0F

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 3D36 (from G. stearothermophilus, complex with Sda)

• UniProt: Q08430

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: kinB-kapB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: