Difference between revisions of "GltC"
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[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 10:10, 23 April 2010
- Description: Transcriptional activator of the gltA-gltB operon. Activates expression of the operon in the absence of arginine.
Gene name | gltC |
Synonyms | |
Essential | No |
Product | transcriptional regulator (LysR family) |
Function | positive regulation of the glutamate synthase operon (gltAB) |
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
MW, pI | 33.9 kDa, 5.62 |
Gene length, protein length | 900 bp, 300 amino acids |
Immediate neighbours | gltA, proJ |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU18460
Phenotypes of a mutant
gltC mutants are auxotrophic for glutamate.
Database entries
- DBTBS entry: [1]
- SubtiList entry:[2]
Additional information
The protein
Basic information/ Evolution
- Protein family: LysR familyPubMed
- Paralogous protein(s): none, but there are 19 members of the LysR family in B. subtilis
Extended information on the protein
- Kinetic information:
- Domains: DNA-binding helix-turn-helix motif: AA 18 ... 37
- Modification:
- Cofactor(s):
- Effectors of protein activity: 2-oxoglutarate stimulates transcription activation, glutamate inhibits transcription activation PubMed
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P20668
- KEGG entry: [3]
Additional information
Expression and regulation
- Regulation: autoregulation by GltC PubMed
- Regulatory mechanism: autorepression PubMed
- Database entries: DBTBS
- Additional information:
Biological materials
- Mutant: GP344 (erm), GP738 (spc) (available in Stülke lab)
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody: available in Stülke lab
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References
Reviews
Sabine Brantl, Andreas Licht
Characterisation of Bacillus subtilis transcriptional regulators involved in metabolic processes.
Curr Protein Pept Sci: 2010, 11(4);274-91
[PubMed:20408793]
[WorldCat.org]
[DOI]
(I p)
Original Publications