YxaL

• Description: similar to Ser/Thr kinase, increases the processivity of the PcrA helicase
  
  

Gene name	yxaL
Synonyms	yxaK
Essential	no
Product	unknown
Function	unknown
MW, pI	43 kDa, 6.379
Gene length, protein length	1230 bp, 410 aa
Immediate neighbours	yxaM, yxaJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39940

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: YxaL-PcrA PubMed

• Localization: extracellular (signal peptide), major constituent of the secretome PubMed

Database entries

• Structure:

• UniProt: P42111

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yxaJ-yxaL PubMed

• Sigma factor:

• Regulation:
  • repressed by Rok PubMed
  • repressed during logarithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • Rok: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • pGP392 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP824, available in Stülke lab

• 'lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: