Difference between revisions of "PbpG"

From SubtiWiki
Jump to: navigation, search
(Expression and regulation)
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''pbpG'' (according to [http://dbtbs.hgc.jp/COG/prom/ywhE.html DBTBS])
  
* '''[[Sigma factor]]:''' [[SigF]], [[SigG]] {{PubMed|16497325,15758244}}
+
* '''[[Sigma factor]]:''' [[SigF]], [[SigG]] {{PubMed|16497325,15758244,10767540}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** expressed during sporulation in the forespore ([[SigF]], [[SigG]]) {{PubMed|16497325,15758244}}
+
** expressed during sporulation in the forespore ([[SigF]], [[SigG]]) {{PubMed|16497325,15758244,10767540}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  

Revision as of 20:12, 3 February 2010

Gene name pbpG
Synonyms ywhE
Essential no
Product penicillin-binding protein 2d
Function bifunctional glucosyltransferase/ transpeptidase
MW, pI 71 kDa, 9.392
Gene length, protein length 1941 bp, 647 aa
Immediate neighbours speE, ywhD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwhE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU37510

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: pbpG (according to DBTBS)
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

D C McPherson, A Driks, D L Popham
Two class A high-molecular-weight penicillin-binding proteins of Bacillus subtilis play redundant roles in sporulation.
J Bacteriol: 2001, 183(20);6046-53
[PubMed:11567005] [WorldCat.org] [DOI] (P p)

L B Pedersen, K Ragkousi, T J Cammett, E Melly, A Sekowska, E Schopick, T Murray, P Setlow
Characterization of ywhE, which encodes a putative high-molecular-weight class A penicillin-binding protein in Bacillus subtilis.
Gene: 2000, 246(1-2);187-96
[PubMed:10767540] [WorldCat.org] [DOI] (P p)