Difference between revisions of "GltC"
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− | * '''Description:''' Transcriptional activator of the '' | + | * '''Description:''' Transcriptional activator of the ''[[gltA]]-[[gltB]]'' operon. Activates expression of the operon in the absence of arginine. <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || No | |style="background:#ABCDEF;" align="center"| '''Essential''' || No | ||
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− | |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator (LysR family) | + | |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator ([[LysR family]]) |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || positive regulation <br/>of the glutamate synthase operon (''gltAB'') | |style="background:#ABCDEF;" align="center"|'''Function''' || positive regulation <br/>of the glutamate synthase operon (''gltAB'') | ||
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* '''Catalyzed reaction/ biological activity:''' transcription activation of the ''[[gltA]]-[[gltB]]'' operon [http://www.ncbi.nlm.nih.gov/sites/entrez/2548995 PubMed] | * '''Catalyzed reaction/ biological activity:''' transcription activation of the ''[[gltA]]-[[gltB]]'' operon [http://www.ncbi.nlm.nih.gov/sites/entrez/2548995 PubMed] | ||
− | * '''Protein family:''' | + | * '''Protein family:''' [[LysR family]][http://www.ncbi.nlm.nih.gov/sites/entrez/2548995 PubMed] |
* '''Paralogous protein(s):''' none, but there are 19 members of the LysR family in ''B. subtilis'' | * '''Paralogous protein(s):''' none, but there are 19 members of the LysR family in ''B. subtilis'' |
Revision as of 23:40, 17 November 2009
- Description: Transcriptional activator of the gltA-gltB operon. Activates expression of the operon in the absence of arginine.
Gene name | gltC |
Synonyms | |
Essential | No |
Product | transcriptional regulator (LysR family) |
Function | positive regulation of the glutamate synthase operon (gltAB) |
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
MW, pI | 33.9 kDa, 5.62 |
Gene length, protein length | 900 bp, 300 amino acids |
Immediate neighbours | gltA, proJ |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU18460
Phenotypes of a mutant
gltC mutants are auxotrophic for glutamate.
Database entries
- DBTBS entry: [1]
- SubtiList entry:[2]
Additional information
The protein
Basic information/ Evolution
- Protein family: LysR familyPubMed
- Paralogous protein(s): none, but there are 19 members of the LysR family in B. subtilis
Extended information on the protein
- Kinetic information:
- Domains: DNA-binding helix-turn-helix motif: AA 18 ... 37
- Modification:
- Cofactor(s):
- Effectors of protein activity: 2-oxoglutarate stimulates transcription activation, glutamate inhibits transcription activation PubMed
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P20668
- KEGG entry: [3]
Additional information
Expression and regulation
- Regulation: autoregulation by GltC PubMed
- Regulatory mechanism: autorepression PubMed
- Database entries: DBTBS
- Additional information:
Biological materials
- Mutant: GP344 (erm), GP738 (spc) (available in Stülke lab)
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody: available in Stülke lab
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References