Difference between revisions of "LiaS"
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|style="background:#ABCDEF;" align="center"| '''Product''' || two-component sensor kinase (NarL family) | |style="background:#ABCDEF;" align="center"| '''Product''' || two-component sensor kinase (NarL family) | ||
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || control of LiaR activity in response to bacitracin | + | |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[LiaR]] activity in response to bacitracin |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 7.333 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 7.333 | ||
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' |
* '''Expression vector:''' | * '''Expression vector:''' | ||
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[[John Helmann]], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage] | [[John Helmann]], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage] | ||
| − | |||
=Your additional remarks= | =Your additional remarks= | ||
Revision as of 21:41, 17 November 2009
- Description: two-component sensor kinase, response to bacitracin
| Gene name | liaS |
| Synonyms | yvqE |
| Essential | no |
| Product | two-component sensor kinase (NarL family) |
| Function | control of LiaR activity in response to bacitracin |
| MW, pI | 40 kDa, 7.333 |
| Gene length, protein length | 1080 bp, 360 aa |
| Immediate neighbours | liaR, liaF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU33090
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O32198
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor: SigA
liaS: constitutive
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
John Helmann, Cornell University, USA Homepage
Your additional remarks
References
