GltC

• Description: Transcriptional activator of the gltAB operon. Activates expression of the operon in the absence of arginine.
  
  

Gene name	gltC
Synonyms
Essential	No
Product	transcriptional regulator (LysR family)
Function	positive regulation of the glutamate synthase operon (gltAB)
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	33.9 kDa, 5.62
Gene length, protein length	900 bp, 300 amino acids
Immediate neighbours	gltA, proJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU18460

Phenotypes of a mutant

gltC mutants are auxotrophic for glutamate.

Database entries

• DBTBS entry: [1]

• SubtiList entry:[2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription activation of the gltA-gltB operon PubMed

• Protein family: glutamate synthase family (according to Swiss-Prot) LysR-type transcription regulator PubMed

• Paralogous protein(s): none, but there are 19 members of the LysR family in B. subtilis

Extended information on the protein

• Kinetic information:

• Domains: DNA-binding helix-turn-helix motif: AA 18 ... 37

• Modification:

• Cofactor(s):

• Effectors of protein activity: 2-oxoglutarate stimulates transcription activation, glutamate inhibits transcription activation PubMed

• Interactions:
  • GltC-RocG, This interaction takes place in the presence of glutamate. It prevents the transcription activation of the gltA-gltB operon. Note that RocG expression is strongly regulated by carbon and nitrogen sources, respectively. PubMed

• Localization:

Database entries

• Structure:

• UniProt: P20668

• KEGG entry: [3]

Expression and regulation

• Operon: gltC

• Sigma factor: SigA

• Regulation: autoregulation by GltC

• Regulatory mechanism: autorepression

• Database entries: DBTBS

• Additional information:

Biological materials

• Mutant: GP344 (erm), GP738 (spc) (available in Stülke lab)

• Expression vector:
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP903, available in Stülke lab
  • for expression, purification in E. coli with C-terminal Strep-tag, in pET3C: pGP951, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody: available in Stülke lab

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Jörg Stülke, University of Göttingen, Germany Homepage