TagT
- Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid
Gene name | ywtF |
Synonyms | |
Essential | no |
Product | phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan |
Function | transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan |
Gene expression levels in SubtiExpress: tagT | |
Interactions involving this protein in SubtInteract: TagT | |
MW, pI | 35 kDa, 9.554 |
Gene length, protein length | 966 bp, 322 aa |
Immediate neighbours | ywtG, ywtE |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU35840
Phenotypes of a mutant
- a tagT mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed
Database entries
- BsubCyc: BSU35840
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: LytR-Cps2a-Psr family PubMed
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): Mg(2+) PubMed
- Effectors of protein activity:
Database entries
- BsubCyc: BSU35840
- UniProt: Q7WY78
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: ywtF PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Stephanie Brown, John P Santa Maria, Suzanne Walker
Wall teichoic acids of gram-positive bacteria.
Annu Rev Microbiol: 2013, 67;313-36
[PubMed:24024634]
[WorldCat.org]
[DOI]
(I p)
Original publications
Alice Eberhardt, Christopher N Hoyland, Daniela Vollmer, Stephanie Bisle, Robert M Cleverley, Ola Johnsborg, Leiv S Håvarstein, Richard J Lewis, Waldemar Vollmer
Attachment of capsular polysaccharide to the cell wall in Streptococcus pneumoniae.
Microb Drug Resist: 2012, 18(3);240-55
[PubMed:22432711]
[WorldCat.org]
[DOI]
(I p)
Yoshikazu Kawai, Jon Marles-Wright, Robert M Cleverley, Robyn Emmins, Shu Ishikawa, Masayoshi Kuwano, Nadja Heinz, Nhat Khai Bui, Christopher N Hoyland, Naotake Ogasawara, Richard J Lewis, Waldemar Vollmer, Richard A Daniel, Jeff Errington
A widespread family of bacterial cell wall assembly proteins.
EMBO J: 2011, 30(24);4931-41
[PubMed:21964069]
[WorldCat.org]
[DOI]
(I e)
Judith Hübscher, Lucas Lüthy, Brigitte Berger-Bächi, Patricia Stutzmann Meier
Phylogenetic distribution and membrane topology of the LytR-CpsA-Psr protein family.
BMC Genomics: 2008, 9;617
[PubMed:19099556]
[WorldCat.org]
[DOI]
(I e)
Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862]
[WorldCat.org]
[DOI]
(I p)
Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421]
[WorldCat.org]
[DOI]
(P p)
E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933]
[WorldCat.org]
[DOI]
(P p)