mrfA

mrfA
168

ATP-dependent helicase, repair of mitomycin C-induced DNA lesions, part of a novel nucleotide excision repair pathway

Locus
BSU_22220
Molecular weight
84.40 kDa
Isoelectric point
6.16
Protein length
749 aaSequenceBlast
Gene length
2250 bpSequenceBlast
Function
repair of mitomycin C-induced DNA lesions
Product
3′-to-5′ DNA helicase
Essential
no
Synonyms
mrfA, yprA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1205 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,334,581  2,336,830
Phenotypes of a mutant
sensitive to mitomycin C PubMed
The protein
Catalyzed reaction/ biological activity
3′-to-5′ DNA helicase PubMed
Protein family
helicase family (according to UniProt)
Domains
Helicase ATP-binding domain (RecA-like) (aa 40-250) PubMed
Helicase C-terminal domain (RecA-like) (aa 251-423) PubMed
Winged helix domain (aa 424-490) PubMed
Zn2+-binding domain (aa 631-749) PubMed
Cofactors
Zn2+ PubMed
Structure
6ZNS (PDB) (apo-MrfA) PubMed
6ZNP (PDB) (MrfA-DNA) PubMed
P50830 (AlphaFold)
Expression and Regulation
Operons
Genes
mrfA-mrfB-cotD
Description
PubMed
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mrfA→cotD

2025-03-16 16:00:43

Jstuelk

66

14a2bf6c0f2fb4d70fe21c4e69da61c2a7c1ca91

729017EE549CDEA9A9C262C8E1D54EEDD385E33D

Biological materials
Mutant
MGNA-A412 (yprA::erm), available at the NBRP B. subtilis, Japan
BKE22220 (mrfA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTTTACACCTCTTTGT,  downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC
BKK22220 (mrfA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTTTACACCTCTTTGT,  downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC
References
Reviews
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Research papers
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Time of last update: 2025-04-02 10:48:57

Author of last update: Jstuelk